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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2021.06.048
|2 doi
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|a pubmed24n1092.xml
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|a (DE-627)NLM327614536
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|a (NLM)34214779
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|a (PII)S0981-9428(21)00361-2
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a González-Thuillier, Irene
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Sunflower (Helianthus annuus) fatty acid synthase complex
|b β-Ketoacyl-[acyl carrier protein] reductase genes
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 07.09.2021
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|a Date Revised 07.09.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
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|a Fatty acids play many roles in plants, but the function of some key genes involved in fatty acid biosynthesis in plant development are not yet properly understood. Here, we clone two β-ketoacyl-[ACP] reductase (KAR) genes from sunflower, HaKAR1 and HaKAR2, and characterize their functional roles. The enzymes cloned were the only two copies present in the sunflower genome. Both displayed a high degree of similarity, but their promoters infer different regulation. The two sunflower KAR genes were constitutively expressed in all tissues examined, being maximum in developing cotyledons at the start of oil synthesis. Over-expression of HaKAR1 in E. coli changed the fatty acid composition by promoting the elongation of C16:0 to C18:0 fatty acids. The enzymatic characterization of HaKAR1 revealed similar kinetic parameters to homologues from other oil accumulating species. The results point to a partially functional redundancy between HaKAR1 and HaKAR2. This study clearly revealed that these genes play a prominent role in de novo fatty acids synthesis in sunflower seeds
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|a Journal Article
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|a Fatty acid synthase (FAS)
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|a Helianthus annuus
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|a Oil biosynthesis
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|a Short-chain dehydrogenases/reductases (SDRs) family
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| 650 |
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|a Substrate specificity
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| 650 |
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|a WRINKLED1 (WRI1)
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| 650 |
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|a β-ketoacyl-[ACP] reductase (KAR)
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| 650 |
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|a Acyl Carrier Protein
|2 NLM
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| 650 |
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7 |
|a Fatty Acids
|2 NLM
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| 650 |
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|a 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase
|2 NLM
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| 650 |
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7 |
|a EC 1.1.1.100
|2 NLM
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| 650 |
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|a Fatty Acid Synthases
|2 NLM
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| 650 |
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|a EC 2.3.1.85
|2 NLM
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| 700 |
1 |
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|a Venegas-Calerón, Mónica
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Moreno-Pérez, Antonio J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Salas, Joaquín J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Garcés, Rafael
|e verfasserin
|4 aut
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| 700 |
1 |
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|a von Wettstein-Knowles, Penny
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martínez-Force, Enrique
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 166(2021) vom: 01. Sept., Seite 689-699
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:166
|g year:2021
|g day:01
|g month:09
|g pages:689-699
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2021.06.048
|3 Volltext
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|d 166
|j 2021
|b 01
|c 09
|h 689-699
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