Biosynthesis of polyhydroxyalkanoate from food waste oil by Pseudomonas alcaligenes with simultaneous energy recovery from fermentation wastewater

Copyright © 2021 Elsevier Ltd. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Waste management (New York, N.Y.). - 1999. - 131(2021) vom: 15. Juli, Seite 268-276
1. Verfasser: Pan, Lanjia (VerfasserIn)
Weitere Verfasser: Li, Jie, Wang, Ruming, Wang, Yu, Lin, Qinghuai, Li, Chunxing, Wang, Yin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Waste management (New York, N.Y.)
Schlagworte:Journal Article Anaerobic fermentation Biodegradable plastic Food waste oil Pseudomonas alcaligenes Polyhydroxyalkanoates Waste Water
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520 |a Bioconversion of food waste oil (FWO) into biodegradable plastic is a promising method for converting waste into high-value products. In this study, a strain (Pseudomonas sp. H3) was isolated for polyhydroxyalkanoate (PHA) synthesis from FWO. After 72 h of cultivation with 20 g/L of FWO, the high cell dry weight (CDW) of 3.6 g/L, PHA yield of 2.4 g/L, and PHA content of 65 wt% were obtained under the optimal temperature (25 °C) and inoculum amount (6% (v/v)). Fed-batch fermentation was conducted in a 5 L bioreactor with a maximum CDW of 16 g/L, PHA content of 54 wt%, and PHA productivity of 0.23 g/(L·h) after 36 h. The PHA had a molecular weight of 54 782 Da and a low polydispersity index of 1.41 with glass transition, melting, and degradation temperatures of -20 °C, 34 °C, and 210 °C, respectively. To further utilize the wastewater after PHA production, anaerobic digestion was employed for CH4 production, and the CH4 yield was 284 mL/g volatile solids. Microbial community analysis showed that the abundance of acetate-oxidizing bacteria and Methanobacterium significantly increased during anaerobic digestion. This study describes a new strain for the economical synthesis of biodegradable plastics and presents a novel framework for fully utilizing FWO with the production of PHA and CH4 
650 4 |a Journal Article 
650 4 |a Anaerobic fermentation 
650 4 |a Biodegradable plastic 
650 4 |a Food waste oil 
650 4 |a Pseudomonas alcaligenes 
650 7 |a Polyhydroxyalkanoates  |2 NLM 
650 7 |a Waste Water  |2 NLM 
700 1 |a Li, Jie  |e verfasserin  |4 aut 
700 1 |a Wang, Ruming  |e verfasserin  |4 aut 
700 1 |a Wang, Yu  |e verfasserin  |4 aut 
700 1 |a Lin, Qinghuai  |e verfasserin  |4 aut 
700 1 |a Li, Chunxing  |e verfasserin  |4 aut 
700 1 |a Wang, Yin  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Waste management (New York, N.Y.)  |d 1999  |g 131(2021) vom: 15. Juli, Seite 268-276  |w (DE-627)NLM098197061  |x 1879-2456  |7 nnas 
773 1 8 |g volume:131  |g year:2021  |g day:15  |g month:07  |g pages:268-276 
856 4 0 |u http://dx.doi.org/10.1016/j.wasman.2021.06.008  |3 Volltext 
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