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20231225195310.0 |
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cr uuu---uuuuu |
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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/nph.17554
|2 doi
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|a pubmed24n1089.xml
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|a (DE-627)NLM326762620
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|a (NLM)34128546
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Di, Dong-Wei
|e verfasserin
|4 aut
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| 245 |
1 |
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|a WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole-3-acetic acid-conjugating genes and by inhibiting ammonium efflux in the root elongation zone
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 09.09.2021
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| 500 |
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|a Date Revised 09.09.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.
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|a Ammonium (NH4+ ) is toxic to root growth in most plants, even at moderate concentrations. Transcriptional regulation is one of the most important mechanisms in the response of plants to NH4+ toxicity, but the nature of the involvement of transcription factors (TFs) in this regulation remains unclear. Here, RNA-seq analysis was performed on Arabidopsis roots to screen for ammonium-responsive TFs. WRKY46, the member of the WRKY transcription factor family most responsive to NH4+ , was selected. We defined the role of WRKY46 using mutation and overexpression assays, and characterized the regulation of NUDX9 and indole-3-acetic acid (IAA)-conjugating genes by WRKY46 via yeast one-hybrid and electrophoretic mobility shift assays and chromatin immunoprecipitation-quantitative real-time polymerase chain reaction (ChIP-qPCR). Knockout of WRKY46 increased, while overexpression of WRKY46 decreased, NH4+ -suppression of the primary root. WRKY46 is shown to directly bind to the promoters of the NUDX9 and IAA-conjugating genes (GH3.1, GH3.6, UGT75D1, UGT84B2) and to inhibit their transcription, thus positively regulating free IAA content and stabilizing protein N-glycosylation, leading to an inhibition of NH4+ efflux in the root elongation zone (EZ). We identify TF involvement in the regulation of NH4+ efflux in the EZ, and show that WRKY46 inhibits NH4+ efflux by negative regulation of NUDX9 and IAA-conjugating genes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a NH4+ efflux
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| 650 |
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|a NH4+ toxicity
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| 650 |
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4 |
|a WRKY46
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| 650 |
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|a free IAA
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| 650 |
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4 |
|a protein N-glycosylation
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| 650 |
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4 |
|a root growth inhibition
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| 650 |
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|a Ammonium Compounds
|2 NLM
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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7 |
|a Indoleacetic Acids
|2 NLM
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| 650 |
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7 |
|a indoleacetic acid
|2 NLM
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| 650 |
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7 |
|a 6U1S09C61L
|2 NLM
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| 700 |
1 |
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|a Sun, Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Meng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Jingjing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kronzucker, Herbert J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Shuang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chu, Jinfang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shi, Weiming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Guangjie
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 232(2021), 1 vom: 15. Okt., Seite 190-207
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
8 |
|g volume:232
|g year:2021
|g number:1
|g day:15
|g month:10
|g pages:190-207
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/nph.17554
|3 Volltext
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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| 952 |
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|d 232
|j 2021
|e 1
|b 15
|c 10
|h 190-207
|