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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/nph.17535
|2 doi
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|a pubmed25n1087.xml
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|a (NLM)34096619
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Chen, Hongyu
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Cytoplasmic and nuclear Sw-5b NLR act both independently and synergistically to confer full host defense against tospovirus infection
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 26.08.2021
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.
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|a Plant intracellular nucleotide-binding leucine-rich repeat (NLR) receptors play critical roles in mediating host immunity to pathogen attack. We use tomato Sw-5b::tospovirus as a model system to study the specific role of the compartmentalized plant NLR in dictating host defenses against the virus at different infection steps. We demonstrated here that tomato NLR Sw-5b distributes to the cytoplasm and nucleus, respectively, to play different roles in inducing host resistances against tomato spotted wilt orthotospovirus (TSWV) infection. The cytoplasmic-enriched Sw-5b induces a strong cell death response to inhibit TSWV replication. This host response is, however, insufficient to block viral intercellular and long-distance movement. The nuclear-enriched Sw-5b triggers a host defense that weakly inhibits viral replication but strongly impedes virus intercellular and systemic movement. Furthermore, the cytoplasmic and nuclear Sw-5b act synergistically to dictate a full host defense of TSWV infection. We further demonstrated that the extended N-terminal Solanaceae domain (SD) of Sw-5b plays critical roles in cytoplasm/nucleus partitioning. Sw-5b NLR controls its cytoplasm localization. Strikingly, the SD but not coil-coil domain is crucial for Sw-5b receptor to import into the nucleus to trigger the immunity. The SD was found to interact with importins. Silencing both importin α and β expression disrupted Sw-5b nucleus import and host immunity against TSWV systemic infection. Collectively, our findings suggest that Sw-5b bifurcates disease resistances by cytoplasm/nucleus partitioning to block different infection steps of TSWV. The findings also identified a new regulatory role of extra domain of a plant NLR in mediating host innate immunity
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a cell-to-cell movement and long-distance movement
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|a cytoplasm
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| 650 |
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4 |
|a nuclear
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|a nucleotide-binding leucine-rich repeats (NLRs)
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| 650 |
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4 |
|a plant innate immunity
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| 650 |
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4 |
|a replication
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| 650 |
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4 |
|a tomato spotted wilt virus
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| 700 |
1 |
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|a Qian, Xin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Xiaojiao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Tongqing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Feng, Mingfeng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Jing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Ruixiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hong, Hao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zheng, Ying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mei, Yuzhen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shen, Danyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhu, Min
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ding, Xin Shun
|e verfasserin
|4 aut
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| 700 |
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|a Tao, Xiaorong
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1984
|g 231(2021), 6 vom: 17. Sept., Seite 2262-2281
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
1 |
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|g volume:231
|g year:2021
|g number:6
|g day:17
|g month:09
|g pages:2262-2281
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| 856 |
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|u http://dx.doi.org/10.1111/nph.17535
|3 Volltext
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|d 231
|j 2021
|e 6
|b 17
|c 09
|h 2262-2281
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