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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202101993
|2 doi
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|a pubmed24n1481.xml
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|a eng
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| 100 |
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|a Shen, Jie
|e verfasserin
|4 aut
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|a Traceable Nano-Biohybrid Complexes by One-Step Synthesis as CRISPR-Chem Vectors for Neurodegenerative Diseases Synergistic Treatment
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|c 2021
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 24.07.2024
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|a Date Revised 24.07.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 Wiley-VCH GmbH.
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|a Abnormal protein aggregations are essential pathological features of neurodegenerative diseases. Eliminating while inhibiting the regeneration of these protein aggregates is considered an effective treatment strategy. Herein, the CRISPR/Cas9 gene-editing tool is employed to inhibit the regeneration of disease-related proteins, while chemical drugs are applied to eliminate the proteins that are produced. To efficiently deliver CRISPR-chem drugs into brain lesions, traceable nano-biohybrid complexes (F-TBIO) are constructed by one-step synthesis and CRISPR/Cas9 plasmids (CF-TBIO) are loaded in a controllable manner. CF-TBIO can knock out the BACE1 gene and reduce the burden of amyloid-β, and thereby significantly improve the cognitive abilities of 2xTg-AD mice. In particular, by prolonging the dosing interval, the pathological damage and behavioral abilities of 2xTg-AD mice are still significantly improved. During the therapeutic process, CF-TBIO with a high relaxation rate provides accurate imaging signals in the complex brain physiological environment. The finding shows that CF-TBIO has great potential to serve as a CRISPR-chem drug-delivery platform for neurodegenerative diseases therapy
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|a Journal Article
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|a CRISPR-chem vectors
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|a CRISPR/Cas9
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|a neurodegenerative diseases synergistic treatment
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|a one-step synthesis
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|a traceable nano-biohybrid complexes
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|a Amyloid beta-Peptides
|2 NLM
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| 700 |
1 |
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|a Lu, Zhiguo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Jianze
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hao, Qiulian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ji, Weihong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Yanyue
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Peng, Huan
|e verfasserin
|4 aut
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| 700 |
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|a Zhao, Ruichen
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Jun
|e verfasserin
|4 aut
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| 700 |
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|a Li, Yan
|e verfasserin
|4 aut
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| 700 |
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|a Shi, Zhuyan
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Xin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 33(2021), 27 vom: 15. Juli, Seite e2101993
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|g volume:33
|g year:2021
|g number:27
|g day:15
|g month:07
|g pages:e2101993
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|u http://dx.doi.org/10.1002/adma.202101993
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