A new P450 involved in the furanocoumarin pathway underlies a recent case of convergent evolution

A new P450 involved in the furanocoumarin pathway underlies a recent case of convergent evolution

© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 231(2021), 5 vom: 19. Sept., Seite 1923-1939
1. Verfasser: Villard, Cloé (VerfasserIn)
Weitere Verfasser: Munakata, Ryosuke, Kitajima, Sakihito, van Velzen, Robin, Schranz, Michael Eric, Larbat, Romain, Hehn, Alain
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Ficus carica (fig tree) convergent evolution cytochrome P450 furanocoumarins marmesin synthase Furocoumarins Cytochrome P-450 Enzyme System 9035-51-2
Beschreibung
Zusammenfassung:© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.
Furanocoumarins are phytoalexins often cited as an example to illustrate the arms race between plants and herbivorous insects. They are distributed in a limited number of phylogenetically distant plant lineages, but synthesized through a similar pathway, which raised the question of a unique or multiple emergence in higher plants. The furanocoumarin pathway was investigated in the fig tree (Ficus carica, Moraceae). Transcriptomic and metabolomic approaches led to the identification of CYP76F112, a cytochrome P450 catalyzing an original reaction. CYP76F112 emergence was inquired using phylogenetics combined with in silico modeling and site-directed mutagenesis. CYP76F112 was found to convert demethylsuberosin into marmesin with a very high affinity. This atypical cyclization reaction represents a key step within the polyphenol biosynthesis pathway. CYP76F112 evolutionary patterns suggests that the marmesin synthase activity appeared recently in the Moraceae family, through a lineage-specific expansion and diversification. The characterization of CYP76F112 as the first known marmesin synthase opens new prospects for the use of the furanocoumarin pathway. It also supports the multiple acquisition of furanocoumarin in angiosperms by convergent evolution, and opens new perspectives regarding the ability of cytochromes P450 to evolve new functions related to plant adaptation to their environment
Beschreibung:Date Completed 12.08.2021
Date Revised 12.08.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.17458