Graphene Energy Transfer for Single-Molecule Biophysics, Biosensing, and Super-Resolution Microscopy
© 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
| Veröffentlicht in: | Advanced materials (Deerfield Beach, Fla.). - 1998. - 33(2021), 24 vom: 04. Juni, Seite e2101099 |
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| Weitere Verfasser: | , , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | Advanced materials (Deerfield Beach, Fla.) |
| Schlagworte: | Journal Article DNA origami Förster-type resonance energy transfer biophysics biosensing graphene single molecules super-resolution Graphite 7782-42-5 mehr... |
| Zusammenfassung: | © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH. Graphene is considered a game-changing material, especially for its mechanical and electrical properties. This work exploits that graphene is almost transparent but quenches fluorescence in a range up to ≈40 nm. Graphene as a broadband and unbleachable energy-transfer acceptor without labeling, is used to precisely determine the height of molecules with respect to graphene, to visualize the dynamics of DNA nanostructures, and to determine the orientation of Förster-type resonance energy transfer (FRET) pairs. Using DNA origami nanopositioners, biosensing, single-molecule tracking, and DNA PAINT super-resolution with <3 nm z-resolution are demonstrated. The range of examples shows the potential of graphene-on-glass coverslips as a versatile platform for single-molecule biophysics, biosensing, and super-resolution microscopy |
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| Beschreibung: | Date Completed 24.07.2024 Date Revised 13.10.2024 published: Print-Electronic ErratumIn: Adv Mater. 2021 Oct;33(42):e2105719. doi: 10.1002/adma.202105719. - PMID 34665480 Citation Status MEDLINE |
| ISSN: | 1521-4095 |
| DOI: | 10.1002/adma.202101099 |