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20250301124845.0 |
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231225s2021 xx |||||o 00| ||chi c |
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|a 10.3760/cma.j.cn112140-20201126-01059
|2 doi
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|a pubmed25n1081.xml
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|a (DE-627)NLM324557140
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|a (NLM)33902224
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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| 100 |
1 |
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|a Xiao, H
|e verfasserin
|4 aut
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1 |
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|a Mechanism of scavenger receptor-A in high glucose-induced inflammatory injury of mesangial cells
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| 264 |
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.04.2021
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|a Date Revised 04.12.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a Objective: To investigate the effect of high glucose on scavenger receptor-A (SR-A) in human glomerular mesangial cells (HMC) and explore the mechanism of inflammatory injury mediated by SR-A in HMC cultured in high-glucose medium. Methods: According to the concentration of D-glucose in culture medium, HMC were divided into normal glucose group (5.5 mmol/L) and high glucose group (30 mmol/L), with mannitol group as hypertonic control. High glucose group was transfected with SR-A small interfering RNA (siSR-A) and the transfection control (siNC) group were set up. Western blotting technology was used to detect the levels of SR-A, NOD-like receptor family pyrin domain-containing 3 (NLRP3), interleukin-1β (IL-1β) protein. Immunofluorescent staining was applied to measure the SR-A in HMC. The mRNA of NLRP3, Caspase-1, IL-1β, FN, ColⅣ, α-SMA and GRP78 were detected by real-time quantitative PCR. The relative activity of Caspase-1 was detected by enzyme method and the concentration of IL-1β in culture medium was detected by enzyme linked immunosorbent assay. Flow cytometry was used to measure the cell cycles of HMC. One-way ANOVA and SNK-q test were used for statistical analysis. Results: The protein level of SR-A in high glucose group was higher than that in normal glucose group and mannitol group (1.23±0.21 vs. 0.68±0.10, 1.23±0.21 vs. 0.78±0.13, all P<0.05). In addition, mean fluorescence intensity of SR-A, protein levels of NLRP3 and IL-1β, mRNA of NLRP3, Caspase-1 and IL-1β, relative activity of Caspase-1 as well as the concentration of IL-1β in high glucose group were all significantly higher than those in normal glucose group and mannitol group (all P<0.05).After transfection induced silencing, SR-A protein in high glucose siNC group was higher than that in high glucose siSR-A group and normal glucose siNC group (1.23±0.10 vs. 0.20±0.01, 1.23±0.10 vs. 0.87±0.01, all P<0.01). In high glucose siNC group, the NLRP3, IL-1β proteins, the NLRP3, Caspase-1 and IL-1β mRNA, all of the mRNA levels of FN, ColⅣ, α-SMA, GRP78 and the proportion of DNA synthesis phase were all higher than those in high glucose siSR-A group and normal glucose siNC group (all P<0.05). Conclusion: High glucose can promote abnormal cell proliferation, increase mesangial matrix production and enhance oxidative stress response through upregulating SR-A expression, and ultimately aggravate cellular inflammatory damage in HMC, which may be associated with NLRP3-Caspase-1-IL-1β pathway regulated by SR-A expression
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|a Journal Article
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|a Endoplasmic Reticulum Chaperone BiP
|2 NLM
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|a HSPA5 protein, human
|2 NLM
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|a Inflammasomes
|2 NLM
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| 650 |
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|a Interleukin-1beta
|2 NLM
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| 650 |
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|a NLR Family, Pyrin Domain-Containing 3 Protein
|2 NLM
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| 650 |
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|a Receptors, Scavenger
|2 NLM
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|a Glucose
|2 NLM
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|a IY9XDZ35W2
|2 NLM
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| 700 |
1 |
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|a Zhang, G F
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, H P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Y X
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Q
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhonghua er ke za zhi = Chinese journal of pediatrics
|d 1960
|g 59(2021), 5 vom: 02. Mai, Seite 393-399
|w (DE-627)NLM136249191
|x 0578-1310
|7 nnas
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| 773 |
1 |
8 |
|g volume:59
|g year:2021
|g number:5
|g day:02
|g month:05
|g pages:393-399
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| 856 |
4 |
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|u http://dx.doi.org/10.3760/cma.j.cn112140-20201126-01059
|3 Volltext
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