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231225s1994 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.1994.tb02994.x
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|a eng
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|a Martin, Francis
|e verfasserin
|4 aut
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|a NH4 + assimilation in the ectomycorrhizal basidiomycete Laccaria bicolor (Maire) Orton, a 15 N-NMR study
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|c 1994
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 20.04.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Nuclear magnetic resonance spectroscopy was used to monitor 15 NH4 + assimilation and amino acid biosynthesis in the ectomycorrhizal basidiomycete Laccaria bicolor (Maire) Orton. (strain S238). In mycelium growing rapidly on 15 NH4 + , [amido-15 N]glutamine was the major 15 N-labelled species. When 15 N-labelled mycelium was transferred into medium containing 14 NH4 + , the resonance for [amino-15 N]glutamine decreased with a half-life of about 3.0 h, whereas the resonance for [amino-15 N]glutamine remained unchanged. Such behaviour is consistent with glutamine synthetase (GS) being the major route of 15 NH4 + assimilation. However, the higher accumulation of [15 N]alanine observed in the presence of the GS inhibitor, methionine sulfoximine, indicated that a part of the glutamate pool was formed by the glutamate dehydrogenase (GDH) pathway. When the mycelium was in stationary phase (i.e. low extracellular NH4 + ), the intramolecular 15 N labelling of glutamine suggested that the GDH and GS pathways were simultaneously assimilating NH4 + . The N supply and the growth stage, therefore, influence the expression of the activities of GDH and GS. The current isotopic data identify other fates of absorbed 15 N: glutamate decarboxylation gives rise to γ-aminobutyrate; transamination between glutamate and pyruvate yields alanine; and arginine accumulates. It is concluded that GS is the main pathway of primary assimilation of NH4 + in L. bicolor, but GDH may also contribute significantly to this process
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|a Journal Article
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|a Laccaria bicolor
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|a N assimilation
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|a NMR
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|a ectomycorrhizal fungus
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|a glutamate dehydrogenase
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|a glutamine
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|a glutamine synthetase
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|a γ-aminobutyrate
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|a Cõté, Richard
|e verfasserin
|4 aut
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|a Canet, Daniel
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 128(1994), 3 vom: 20. Nov., Seite 479-485
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|x 1469-8137
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|g volume:128
|g year:1994
|g number:3
|g day:20
|g month:11
|g pages:479-485
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