Effect of some triazole fungicides on intracellular pH and on cell membrane permeability in leaves of Elodea densa (Planch.) Casp
Recent data obtained in this laboratory have shown that a number of triazole fungicides induce in leaves of barley and Elodea densa an early release of solutes and an inhibition of H+ extrusion. This paper deals with the early effects of two triazoles, penconazole and flusilazol, on intracellular pH...
| Veröffentlicht in: | The New phytologist. - 1979. - 117(1991), 3 vom: 20. März, Seite 431-437 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1991
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Elodea densa intracellular pH membrane permeability triazoles |
| Zusammenfassung: | Recent data obtained in this laboratory have shown that a number of triazole fungicides induce in leaves of barley and Elodea densa an early release of solutes and an inhibition of H+ extrusion. This paper deals with the early effects of two triazoles, penconazole and flusilazol, on intracellular pH and plasmamembrane permeability in Elodea densa leaves. Different effects were found at low (0.1-0.15 mM) or at high (0.3-0.5 mM) triazole concentrations. At the low concentrations the triazoles induced a marked intracellular acidification (corresponding to an acidification of both the cytosol and the vacuole) as evaluated by the weak acid and weak base distribution method. For higher triazole concentrations (0.3-0.5 mM) the intracellular pH became very close to that of the external medium (pH 5.5), and cytosolic and vacuolar pH measurements became unreliable. Triazoles at concentrations higher than 0.1-0.15 mM induced an early and very marked increase in net efflux of K+ and Cl- . At these 'toxic' concentrations the sorbitol-permeable space (apparent free space) was increased from about 20% to more than 90%. These results suggest that triazoles at low 'sub-toxic' concentrations inhibit some mechanism involved in the regulation of intracellular pH (possibly the plasmalemma proton pump), whilst at higher concentrations they induce a non-specific increase in passive permeability of the plasmamembrane |
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| Beschreibung: | Date Revised 20.04.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/j.1469-8137.1991.tb00007.x |