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231225s1989 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.1989.tb02408.x
|2 doi
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|a eng
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|a Murray, M B
|e verfasserin
|4 aut
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|a Quantification of frost damage in plant tissues by rates of electrolyte leakage
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|c 1989
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.04.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a A method for assessing frost hardiness of plant tissues [using shoots of Picea rubens Sarg. syn P. rubra (Du Roi) Link] has been developed based upon the rate of electrolyte leakage from shoots immersed in distilled water after exposure to a range of freezing treatments. The relationship between conductivity (the electrolyte concentration in solution) and time has been shown to follow an asymptotic curve, which may be represented by a first-order equation: Ct -Co = Cauto -Co - C-kt ) where C1 is the conductivity at time t, Co is the initial conductivity, Cauto is the conductivity after autoclaving and k is the first-order rate constant (units time-1 ). The rate of electrolyte leakage (k) varies directly with the extent of tissue damage. In P. rebens a rate of 0-4%, h-1 distinguished between shoots which eventually died, and shoots which remained alive. A minimum of 3 conductivity measurements (after 1 day, 5 days and after autoclaving) is required for a reliable estimate of k. This objective, quantitative method of assessing frost hardiness may therefore be used directly to estimate LT50 values within a population
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|a Journal Article
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|a Frost damage
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|a conductivity method
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|a electrolyte leakage
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| 650 |
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|a red spruce
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|a Cape, J N
|e verfasserin
|4 aut
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|a Fowler, D
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 113(1989), 3 vom: 20. Nov., Seite 307-311
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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|g volume:113
|g year:1989
|g number:3
|g day:20
|g month:11
|g pages:307-311
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|u http://dx.doi.org/10.1111/j.1469-8137.1989.tb02408.x
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|d 113
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|h 307-311
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