15 N NUCLEAR MAGNETIC RESONANCE STUDY OF AMMONIUM ION ASSIMILATION BY LEMNA GIBBA L
15 N Nuclear magnetic resonance (NMR) spectroscopy was used to follow nitrogen assimilation and amino acid production in Lemna gibba L. exposed to 0.4 mM 15 NH4 Cl solution for 24 h. NMR analysis of the 16 N content of the treated plant tissues (aqueous extract) revealed 15 N incorporation into glut...
| Veröffentlicht in: | The New phytologist. - 1979. - 107(1987), 2 vom: 20. Okt., Seite 341-345 |
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| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1987
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article 15N-NMR GS/GOGAT pathway Lemna gibba MSO and AZA ammonium ion assimilation |
| Zusammenfassung: | 15 N Nuclear magnetic resonance (NMR) spectroscopy was used to follow nitrogen assimilation and amino acid production in Lemna gibba L. exposed to 0.4 mM 15 NH4 Cl solution for 24 h. NMR analysis of the 16 N content of the treated plant tissues (aqueous extract) revealed 15 N incorporation into glutamine (N-amide plus amino-N or amide-N only) and glutamic acid and no detectable free ammonium ion. Methionine sulphoximine (MSO), an inhibitor of glutamine synthetase, at 1.0 mM inhibited completely the incorporation of 15 N. In the presence of 0.5 mM azaserine (AZA), a glutamate synthase inhibitor, the incorporation of 15 N was detected only in the amide group of glutamine. The results confirm the involvement of the glutamine synthetase/glutamate synthase (GS/ GOG AT) pathway in the assimilation of ammonium ions |
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| Beschreibung: | Date Revised 20.04.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/j.1469-8137.1987.tb00186.x |