Identification of premycorrhiza-related plant genes in the association between Quercus robur and Piloderma croceum

•  An in vitro system with micropropagated oaks (Quercus robur) and the ectomycorrhizal fungus Piloderma croceum, which is characterized by a delayed mycorrhiza formation, was used to identify plant transcripts upregulated in the premycorrhizal phase. •  Complementary DNA (cDNA) populations of unino...

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Veröffentlicht in:The New phytologist. - 1979. - 163(2004), 1 vom: 20. Juli, Seite 149-157
1. Verfasser: Krüger, Andrea (VerfasserIn)
Weitere Verfasser: Pescaron Kan-Berghöfer, Tatjana, Frettinger, Patrick, Herrmann, Sylvie, Buscot, François, Oelmüller, Ralf
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Piloderma croceum Quercus robur (common oak) ectomycorrhiza premycorrhiza related plant genes suppression subtractive hybridization
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520 |a •  An in vitro system with micropropagated oaks (Quercus robur) and the ectomycorrhizal fungus Piloderma croceum, which is characterized by a delayed mycorrhiza formation, was used to identify plant transcripts upregulated in the premycorrhizal phase. •  Complementary DNA (cDNA) populations of uninoculated roots and fungal mycelium were subtracted from a cDNA population of inoculated roots. Differential expression was confirmed by reverse Northern and 50 clones for different polypeptides were found to be up-regulated. Twenty-nine clones were investigated in more detail. •  For approximately half of the cDNA fragments no homologies could be identified in databases. The residual fragments code for polypeptides with homologies to known proteins involved in signal perception and transmission, stress responses, metabolism and growth. •  Since many of the identified genes have not yet been described in the context of symbiotic events, their potential roles during early phases of the recognition process are discussed 
650 4 |a Journal Article 
650 4 |a Piloderma croceum 
650 4 |a Quercus robur (common oak) 
650 4 |a ectomycorrhiza 
650 4 |a premycorrhiza related plant genes 
650 4 |a suppression subtractive hybridization 
700 1 |a Pescaron Kan-Berghöfer, Tatjana  |e verfasserin  |4 aut 
700 1 |a Frettinger, Patrick  |e verfasserin  |4 aut 
700 1 |a Herrmann, Sylvie  |e verfasserin  |4 aut 
700 1 |a Buscot, François  |e verfasserin  |4 aut 
700 1 |a Oelmüller, Ralf  |e verfasserin  |4 aut 
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