24-Methyl/methylene sterols increase in monoxenic roots after colonization by arbuscular mycorrhizal fungi
• Characteristic sterols of transformed carrot (Daucus carota) and chicory (Cichorium intybus) roots colonized by different strains of arbuscular mycorrhizal (AM) fungi were identified. • Sterols were extracted, analysed and identified by gas chromatography/mass spectrometry (GC-MS) from monoxenic...
| Veröffentlicht in: | The New phytologist. - 1979. - 163(2004), 1 vom: 20. Juli, Seite 159-167 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Cichorium intybus (chicory) Daucus carota (carrot) Glomus Ri T-DNA-transformed roots monoxenic cultures sterol |
| Zusammenfassung: | • Characteristic sterols of transformed carrot (Daucus carota) and chicory (Cichorium intybus) roots colonized by different strains of arbuscular mycorrhizal (AM) fungi were identified. • Sterols were extracted, analysed and identified by gas chromatography/mass spectrometry (GC-MS) from monoxenic cultures of mycorrhizal and nonmycorrhizal roots. After colonization by Glomus intraradices, Glomus proliferum and Glomus sp., carrot and chicory roots exhibited a significantly higher 24-methyl/methylene sterol content. A correlation was established between the content of the sum of 24-methyl cholesterol, 24-methylene cholesterol and 24-methyl desmosterol. • This study clearly established that the increment of these characteristic sterols is an appropriate indicator of colonization by AM fungi of transformed roots. • Metabolic origin and specificity of these sterols in mycorrhizal roots was researched. The 24-methyl/methylene sterol increase was observed only when the interaction between fungus and plant was completely established and the fungus was present inside the roots |
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| Beschreibung: | Date Revised 20.04.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/j.1469-8137.2004.01075.x |