Analysis of expressed sequence tags from the ectomycorrhizal basidiomycetes Laccaria bicolor and Pisolithus microcarpus

•  In an effort to discover genes that are expressed in the ectomycorrhizal basidiomycetes Laccaria bicolor and Pisolithus microcarpus, and in P. microcarpus/Eucalyptus globulus ectomycorrhizas, we have sequenced 1519 and 1681 expressed sequence tags (ESTs) from L. bicolor and P. microcarpus cDNA li...

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Veröffentlicht in:The New phytologist. - 1979. - 159(2003), 1 vom: 20. Juli, Seite 117-129
1. Verfasser: Peter, Martina (VerfasserIn)
Weitere Verfasser: Courty, Pierre-Emmanuel, Kohler, Annegret, Delaruelle, Christine, Martin, David, Tagu, Denis, Frey-Klett, Pascale, Duplessis, Sébastien, Chalot, Michel, Podila, Gopi, Martin, Francis
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Laccaria bicolor Pisolithus microcarpus ectomycorrhizal basidiomycetes expressed sequence tags (ESTs) gene diversity gene profiling
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520 |a •  In an effort to discover genes that are expressed in the ectomycorrhizal basidiomycetes Laccaria bicolor and Pisolithus microcarpus, and in P. microcarpus/Eucalyptus globulus ectomycorrhizas, we have sequenced 1519 and 1681 expressed sequence tags (ESTs) from L. bicolor and P. microcarpus cDNA libraries. •  Contig analysis resulted in 905 and 806 tentative consensus sequences (unique transcripts) in L. bicolor and P. microcarpus, respectively. For 36% of the ESTs, significant similarities to sequences in databases were detected. The most abundant transcripts showed no similarity to previously identified genes. Sequence redundancy analysis between different developmental stages indicated that several genes were differentially expressed in free-living mycelium and symbiotic tissues of P. microcarpus. •  Based on sequence similarity, 11% of L. bicolor unique transcripts were also detected in P. microcarpus. Similarly, L. bicolor and P. microcarpus shared only a low proportion of common transcripts with other basidiomycetous fungi, such as Pleurotus ostreatus and Agaricus bisporus. Such a low proportion of shared transcripts between basidiomycetes suggests, on the one hand, that the variability of expressed transcripts in different fungi and fungal tissues is considerably high. On the other hand, it might reflect the low number of GenBank entries of basidiomycetous origin and stresses the necessity of an additional sequencing effort. •  The present ESTs provide a valuable resource for future research on the development and functioning of ectomycorrhizas 
650 4 |a Journal Article 
650 4 |a Laccaria bicolor 
650 4 |a Pisolithus microcarpus 
650 4 |a ectomycorrhizal basidiomycetes 
650 4 |a expressed sequence tags (ESTs) 
650 4 |a gene diversity 
650 4 |a gene profiling 
700 1 |a Courty, Pierre-Emmanuel  |e verfasserin  |4 aut 
700 1 |a Kohler, Annegret  |e verfasserin  |4 aut 
700 1 |a Delaruelle, Christine  |e verfasserin  |4 aut 
700 1 |a Martin, David  |e verfasserin  |4 aut 
700 1 |a Tagu, Denis  |e verfasserin  |4 aut 
700 1 |a Frey-Klett, Pascale  |e verfasserin  |4 aut 
700 1 |a Duplessis, Sébastien  |e verfasserin  |4 aut 
700 1 |a Chalot, Michel  |e verfasserin  |4 aut 
700 1 |a Podila, Gopi  |e verfasserin  |4 aut 
700 1 |a Martin, Francis  |e verfasserin  |4 aut 
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