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231225s2004 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2004.01107.x
|2 doi
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|a pubmed24n1080.xml
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|a (DE-627)NLM324282109
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|a (NLM)33873617
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Turrini, A
|e verfasserin
|4 aut
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|a The antifungal Dm-AMP1 protein from Dahlia merckii expressed in Solanum melongena is released in root exudates and differentially affects pathogenic fungi and mycorrhizal symbiosis
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 20.04.2021
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|a published: Print
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|a GENBANK: AAB34972, A26963, EHA105, OD600
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|a Citation Status PubMed-not-MEDLINE
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|a • Transformed aubergine plants constitutively expressing the Dm-AMP1 antimicrobial defensin (from Dahlia merckii) were generated and characterized. • Transgenic plants were selected on kanamycin and screened by polymerase chain reaction analysis. The expression of Dm-AMP1 in plant tissues and its release in root exudates were detected by Western blot analyses. Dm-AMP1 localization was performed by immunohistochemical experiments. • Dm-AMP1 expression ranged from 0.2% to 0.48% of total soluble proteins in primary transformants and from 0.16% to 0.66% in F2 plants. Transformed clones showed resistance to the pathogenic fungus Botrytis cinerea, whose development on leaves was reduced by 36-100%, with respect to controls. The protein was released in root exudates of the transformed plants and was active in reducing the growth of the co-cultured pathogenic fungus Verticillium albo-atrum, whereas it did not interfere with recognition responses and symbiosis establishment by the arbuscular mycorrhizal fungus Glomus mosseae. • Dm-AMP1 transformants may represent a useful model to study the interactions between genetically modified plants and pathogenic fungi or beneficial nontarget microorganisms
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Botrytis cinerea
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| 650 |
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4 |
|a Solanum melongena
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| 650 |
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4 |
|a Verticillium albo-atrum
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| 650 |
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4 |
|a arbuscular mycorrhizal fungi
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| 650 |
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4 |
|a defensin
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| 650 |
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4 |
|a transformed plants
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1 |
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|a Sbrana, C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pitto, L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ruffini Castiglione, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Giorgetti, L
|e verfasserin
|4 aut
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| 700 |
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|a Briganti, R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bracci, T
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Evangelista, M
|e verfasserin
|4 aut
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| 700 |
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|a Nuti, M P
|e verfasserin
|4 aut
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| 700 |
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|a Giovannetti, M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 163(2004), 2 vom: 20. Aug., Seite 393-403
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:163
|g year:2004
|g number:2
|g day:20
|g month:08
|g pages:393-403
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|u http://dx.doi.org/10.1111/j.1469-8137.2004.01107.x
|3 Volltext
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|h 393-403
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