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231225s2003 xx |||||o 00| ||eng c |
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|a 10.1046/j.1469-8137.2003.00841.x
|2 doi
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|a eng
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| 100 |
1 |
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|a Zhao, Jian
|e verfasserin
|4 aut
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|a Peroxidases are involved in biosynthesis and biodegradation of β-thujaplicin in fungal elicitor-treated Cupressus lusitanica cell cultures
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|c 2003
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Revised 20.04.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a • Here, collaboration between peroxidases and H2 O2 in biosynthesis and biodegredation of β-thujaplicin in elicited Cupressus lusitanica was investigated. • The accumulation of a phytoalexin, β-thujaplicin, in C. lusitanica cell cultures can be stimulated by a yeast elicitor. A transient low peak was followed by a high level lasting for 2 d, and then a decrease, while peroxidases were activated to a high level just when amounts of β-thujaplicin decreased. In vitro tests revealed that horseradish peroxidase can transform c. 80% of β-thujaplicin in the presence of H2 O2 , and the culture medium was also able to transform β-thujaplicin. • A transient production of H2 O2 occured in the cell cultures immediately after elicitation, following a increase in NAD(P)H-oxidase activity. This H2 O2 production may mediate the elicitor-induced accumulation of β-thujaplicin, because inhibiting H2 O2 production or removing H2 O2 from the cell cultures suppressed elicitor-induced β-thujaplicin accumulation, while exogenously applied H2 O2 or H2 O2 generation system can stimulate β-thujaplicin accumulation. • Both NAD(P)H oxidase inhibitors and peroxidase inhibitors partially inhibited NAD(P)H-dependent O2 - and H2 O2 production. In-gel assay of peroxidase and superoxide anion synthase activity demonstrated that peroxidase isoforms have NAD(P)H-dependent superoxide anion synthase activity. These results suggest that peroxidases can act as superoxide anion synthases to contribute to genecation of H2 O2 that promotes β-thujaplicin production in elicited C. lusitanica cell cultures
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|a Journal Article
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|a Cupressus lusitanica
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|a H2O2
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|a elicitor
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|a peroxidase
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|a superoxide anion synthase
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| 650 |
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4 |
|a β-thujaplicin
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| 700 |
1 |
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|a Sakai, Kokki
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 159(2003), 3 vom: 20. Sept., Seite 719-731
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:159
|g year:2003
|g number:3
|g day:20
|g month:09
|g pages:719-731
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|u http://dx.doi.org/10.1046/j.1469-8137.2003.00841.x
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