Chitosan induces Ca2+ -mediated programmed cell death in soybean cells
• Chitosan, a component of the cell wall of many fungi, has been widely used to mimic pathogen attack and has been shown to induce several defence responses. • Here we show that low concentrations (50 µg ml-1 ) of chitosan are able to induce an increase in cytosolic Ca2+ concentration ([Ca2+ ]cyt...
| Veröffentlicht in: | The New phytologist. - 1979. - 161(2004), 2 vom: 20. Feb., Seite 557-568 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article calcium caspase 3-like chitosan programmed cell death signal transduction soybean (Glycine max) cells |
| Zusammenfassung: | • Chitosan, a component of the cell wall of many fungi, has been widely used to mimic pathogen attack and has been shown to induce several defence responses. • Here we show that low concentrations (50 µg ml-1 ) of chitosan are able to induce an increase in cytosolic Ca2+ concentration ([Ca2+ ]cyt ), accumulation of H2 O2 in the culture medium, induction of the defence gene chalcone synthase (chs), and cell death in soybean cells (Glycine max). • Chitosan-induced cell death occurred through cytoplasmic shrinkage, chromatin condensation and activation of caspase 3-like protease, suggesting the activation of a programmed cell death (PCD) pathway. Buffering extracellular Ca2+ with the Ca2+ chelator EGTA prevents [Ca2+ ]cyt elevation, H2 O2 production and all downstream PCD features, but not cell death. • Higher doses (200 µg ml-1 ) of chitosan evoked neither Ca2+ transient and H2 O2 production nor caspase 3-like activation, but caused cell death, possibly as a result of plasma membrane disturbance |
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| Beschreibung: | Date Revised 20.04.2021 published: Print-Electronic GENBANK: X53958 Citation Status PubMed-not-MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1046/j.1469-8137.2003.00969.x |