Targeted proteomics to identify cadmium-induced protein modifications in Glomus mosseae-inoculated pea roots

•   Arbuscular mycorrhiza (AM) can increase plant tolerance to heavy metals. A targeted proteomic approach was used to determine the putative identity of some of the proteins induced/modulated by cadmium (Cd) and to analyse the impact of the mycorrhizal process. •   The effect of Cd (100 mg Cd kg-1...

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Veröffentlicht in:The New phytologist. - 1979. - 157(2003), 3 vom: 20. März, Seite 555-567
1. Verfasser: Repetto, Ombretta (VerfasserIn)
Weitere Verfasser: Bestel-Corre, Gwénäelle, Dumas-Gaudot, Eliane, Berta, Graziella, Gianinazzi-Pearson, Vivienne, Gianinazzi, Silvio
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Glomus mosseae MS) Pisum sativum cadmium stress liquid chromatography-tandem mass spectrometry (LC-MS one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses plant biomass protein profiles
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520 |a •   Arbuscular mycorrhiza (AM) can increase plant tolerance to heavy metals. A targeted proteomic approach was used to determine the putative identity of some of the proteins induced/modulated by cadmium (Cd) and to analyse the impact of the mycorrhizal process. •   The effect of Cd (100 mg Cd kg-1  substrate) applied either at planting or 15 d later on two pea (Pisum sativum) genotypes, differing in sensitivity to Cd inoculated or not with the AM fungus Glomus mosseae, was studied at three levels: plant biomass production, development of G. mosseae and root differential protein display with one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses. •   Cd-induced growth inhibition was significantly alleviated by mycorrhiza in the Cd-sensitive genotype. The AM symbiosis modulated the expression of several proteins, identified by liquid chromatography-tandem mass spectrometry, newly induced and upregulated or downregulated by Cd. •   The protective effect of AM symbiosis towards Cd stress was observed in the Cd-sensitive genotype. Our results demonstrate the usefulness of proteomics to better understand the possible role of AM symbiosis in detoxification/response mechanisms towards Cd in pea plants 
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650 4 |a Pisum sativum 
650 4 |a cadmium stress 
650 4 |a liquid chromatography-tandem mass spectrometry (LC-MS 
650 4 |a one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses 
650 4 |a plant biomass 
650 4 |a protein profiles 
700 1 |a Bestel-Corre, Gwénäelle  |e verfasserin  |4 aut 
700 1 |a Dumas-Gaudot, Eliane  |e verfasserin  |4 aut 
700 1 |a Berta, Graziella  |e verfasserin  |4 aut 
700 1 |a Gianinazzi-Pearson, Vivienne  |e verfasserin  |4 aut 
700 1 |a Gianinazzi, Silvio  |e verfasserin  |4 aut 
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