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231225s2003 xx |||||o 00| ||eng c |
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|a 10.1046/j.1469-8137.2003.00682.x
|2 doi
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|a DE-627
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|a eng
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|a Repetto, Ombretta
|e verfasserin
|4 aut
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|a Targeted proteomics to identify cadmium-induced protein modifications in Glomus mosseae-inoculated pea roots
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|c 2003
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 20.04.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a • Arbuscular mycorrhiza (AM) can increase plant tolerance to heavy metals. A targeted proteomic approach was used to determine the putative identity of some of the proteins induced/modulated by cadmium (Cd) and to analyse the impact of the mycorrhizal process. • The effect of Cd (100 mg Cd kg-1 substrate) applied either at planting or 15 d later on two pea (Pisum sativum) genotypes, differing in sensitivity to Cd inoculated or not with the AM fungus Glomus mosseae, was studied at three levels: plant biomass production, development of G. mosseae and root differential protein display with one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses. • Cd-induced growth inhibition was significantly alleviated by mycorrhiza in the Cd-sensitive genotype. The AM symbiosis modulated the expression of several proteins, identified by liquid chromatography-tandem mass spectrometry, newly induced and upregulated or downregulated by Cd. • The protective effect of AM symbiosis towards Cd stress was observed in the Cd-sensitive genotype. Our results demonstrate the usefulness of proteomics to better understand the possible role of AM symbiosis in detoxification/response mechanisms towards Cd in pea plants
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|a Journal Article
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|a Glomus mosseae
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|a MS)
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|a Pisum sativum
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|a cadmium stress
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|a liquid chromatography-tandem mass spectrometry (LC-MS
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|a one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses
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|a plant biomass
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|a protein profiles
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1 |
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|a Bestel-Corre, Gwénäelle
|e verfasserin
|4 aut
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1 |
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|a Dumas-Gaudot, Eliane
|e verfasserin
|4 aut
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700 |
1 |
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|a Berta, Graziella
|e verfasserin
|4 aut
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|a Gianinazzi-Pearson, Vivienne
|e verfasserin
|4 aut
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|a Gianinazzi, Silvio
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 157(2003), 3 vom: 20. März, Seite 555-567
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|x 1469-8137
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|g volume:157
|g year:2003
|g number:3
|g day:20
|g month:03
|g pages:555-567
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