Heterotrimeric G-proteins in plant cell signaling
Heterotrimeric G-proteins, which couple cell surface receptors with internal effectors, are evident in all eukaryotes. Their operation involves receptor activation, GTP/GDP exchange and modulation of effector activity; deactivation occurs by an intrinsic GTPase activity. Structurally, G-proteins com...
| Veröffentlicht in: | The New phytologist. - 1979. - 151(2001), 1 vom: 20. Juli, Seite 165-174 |
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| 1. Verfasser: | |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2001
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article G-protein antisense expression ion channel pathogen phage display phytohormone two hybrid |
| Zusammenfassung: | Heterotrimeric G-proteins, which couple cell surface receptors with internal effectors, are evident in all eukaryotes. Their operation involves receptor activation, GTP/GDP exchange and modulation of effector activity; deactivation occurs by an intrinsic GTPase activity. Structurally, G-proteins comprise three dissimilar subunits; Gα, Gβ and Gγ. The Gα subunit consists of an α-helical and a GTPase domain, the latter is responsible for interaction with Gβγ, receptor and effector. Gβ and Gγ form a tightly associated heterodimer which can also modulate effector activity when released by the activated Gα. Genome sequence and other data suggest that, in plants, there are several (~8-10?) Gα, one or two Gβ and one Gγ. These proteins are expressed throughout the plant, mainly in the plasma membrane and endoplasmic reticulum. In vivo, there is strong evidence for G-protein control of ion channels, particularly K+ , in the response pathways to fungal and bacterial pathogens as well as in some aspects of gibberellin, abscisic acid and auxin signaling pathways. Finally, future prospects for understanding plant G-protein linked signaling will rely on new and emerging technologies; these include antisense suppression, gene knockouts, yeast two-hybrid and phage display molecular approaches, intracellular immunization using recombinant single chain antibodies and expression of peptide encoding minigenes |
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| Beschreibung: | Date Revised 20.04.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1046/j.1469-8137.2001.00172.x |