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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.1c00115
|2 doi
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|a eng
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| 100 |
1 |
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|a Anuganti, Murali
|e verfasserin
|4 aut
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| 245 |
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|a Kinetic Study on Enzymatic Hydrolysis of Cellulose in an Open, Inhibition-Free System
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 18.05.2021
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|a Date Revised 18.05.2021
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Due to the complexity of cellulases and the requirement of enzyme adsorption on cellulose prior to reactions, it is difficult to evaluate their reaction with a general mechanistic scheme. Nevertheless, it is of great interest to come up with an approximate analytic description of a valid model for the purpose of developing an intuitive understanding of these complex enzyme systems. Herein, we used the surface plasmonic resonance method to monitor the action of a cellobiohydrolase by itself, as well as its mixture with a synergetic endoglucanase, on the surface of a regenerated model cellulose film, under continuous flow conditions. We found a phenomenological approach by taking advantage of the long steady state of cellulose hydrolysis in the open, inhibition-free system. This provided a direct and reliable way to analyze the adsorption and reaction processes with a minimum number of fitting parameters. We investigated a generalized Langmuir-Michaelis-Menten model to describe a full set of kinetic results across a range of enzyme concentrations, compositions, and temperatures. The overall form of the equations describing the pseudo-steady-state kinetics of the flow-system shares some interesting similarities with the Michaelis-Menten equation. The use of familiar Michaelis-Menten parameters in the analysis provides a unifying framework to study cellulase kinetics. The strategy may provide a shortcut for approaching a quantitative while intuitive understanding of enzymatic degradation of cellulose from top to bottom. The open system approach and the kinetic analysis should be applicable to a variety of cellulases and reaction systems to accelerate the progress in the field
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 700 |
1 |
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|a Fu, Hailin
|e verfasserin
|4 aut
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|a Ekatan, Stephen
|e verfasserin
|4 aut
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|a Kumar, Challa V
|e verfasserin
|4 aut
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|a Lin, Yao
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 37(2021), 17 vom: 04. Mai, Seite 5180-5192
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:37
|g year:2021
|g number:17
|g day:04
|g month:05
|g pages:5180-5192
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|u http://dx.doi.org/10.1021/acs.langmuir.1c00115
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