A bean epicuticular glycoprotein is present in the extracellular matrices around infection structures of the anthracnose fungus, Colletotrichum lindemuthianum

Monoclonal antibodies were raised to Colletotrichum lindemuthianum (Sacc. & Magn.) Briosi & Cav. infection structures which had been isolated from leaves of Phaseolus vulgaris L. by isopycnic centrifugation and immunomagnetic separation. One of the antibodies, UB29, recognized a carbohydrate...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 134(1996), 4 vom: 18. Dez., Seite 579-585
1. Verfasser: Hutchison, Katie A (VerfasserIn)
Weitere Verfasser: O'Connell, Richard J, Pain, Naomi A, Green, Jonathan R
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Colletotrichum lindemuthianum Phaseolus vulgaris cuticle epicuticular wax extracellular matrix
LEADER 01000naa a22002652 4500
001 NLM324178832
003 DE-627
005 20231225185749.0
007 cr uuu---uuuuu
008 231225s1996 xx |||||o 00| ||eng c
024 7 |a 10.1111/j.1469-8137.1996.tb04923.x  |2 doi 
028 5 2 |a pubmed24n1080.xml 
035 |a (DE-627)NLM324178832 
035 |a (NLM)33863193 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Hutchison, Katie A  |e verfasserin  |4 aut 
245 1 2 |a A bean epicuticular glycoprotein is present in the extracellular matrices around infection structures of the anthracnose fungus, Colletotrichum lindemuthianum 
264 1 |c 1996 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Revised 19.04.2021 
500 |a published: Print 
500 |a Citation Status PubMed-not-MEDLINE 
520 |a Monoclonal antibodies were raised to Colletotrichum lindemuthianum (Sacc. & Magn.) Briosi & Cav. infection structures which had been isolated from leaves of Phaseolus vulgaris L. by isopycnic centrifugation and immunomagnetic separation. One of the antibodies, UB29, recognized a carbohydrate epitope in a 200 kDa glycoprotein present on the surface of conidia, germ-tubes and appressoria when they developed on host tissue. Intracellular hyphae were not labelled. Immunogold labelling showed that the antigen was confined to the extracellular matrices around such structures. No such antigen was detected by immunofluorescence or Western blotting when the fungus developed in vitro. UB29 recognized a glycoprotein of identical Mr located in the epicuticular wax layer of uninoculated bean hypocotyls. The results suggest that a host epicuticular glycoprotein is present in the extracellular matrices of fungal structures during growth on the plant surface 
650 4 |a Journal Article 
650 4 |a Colletotrichum lindemuthianum 
650 4 |a Phaseolus vulgaris 
650 4 |a cuticle 
650 4 |a epicuticular wax 
650 4 |a extracellular matrix 
700 1 |a O'Connell, Richard J  |e verfasserin  |4 aut 
700 1 |a Pain, Naomi A  |e verfasserin  |4 aut 
700 1 |a Green, Jonathan R  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 134(1996), 4 vom: 18. Dez., Seite 579-585  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:134  |g year:1996  |g number:4  |g day:18  |g month:12  |g pages:579-585 
856 4 0 |u http://dx.doi.org/10.1111/j.1469-8137.1996.tb04923.x  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 134  |j 1996  |e 4  |b 18  |c 12  |h 579-585