Characterization and identification of black alder ectomycorrhizas by PCR/RFLP analyses of the rDNA internal transcribed spacer (ITS)

The identity of black alder (Alnus glutinosa (L.) Gaertn.) ectomycorrhizas was investigated using PCR/RFLP analysis of the ITS region from 16 morphotypes sampled at a 60-yr-old black alder stand. A comparison was made with restriction patterns from sporocarps of 28 mycobionts, of which 16 originated...

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Veröffentlicht in:The New phytologist. - 1979. - 137(1997), 2 vom: 18. Okt., Seite 357-369
1. Verfasser: Pritsch, K (VerfasserIn)
Weitere Verfasser: Boyle, H, Munch, J C, Buscot, F
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Alnus glutinosa Diversity ectomycorrhiza identification sporocarp
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245 1 0 |a Characterization and identification of black alder ectomycorrhizas by PCR/RFLP analyses of the rDNA internal transcribed spacer (ITS) 
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520 |a The identity of black alder (Alnus glutinosa (L.) Gaertn.) ectomycorrhizas was investigated using PCR/RFLP analysis of the ITS region from 16 morphotypes sampled at a 60-yr-old black alder stand. A comparison was made with restriction patterns from sporocarps of 28 mycobionts, of which 16 originated from the same stand, the remaining 12 came from two geographically distant alder stands. Eight of the mycorrhizal types could thus be identified, whereas eight mycorrhizal types remained unidentified. The identified mycorrhizas belonged to the genera Russula, Lactarius, Naucoria and Cortinarius. Four of the identified ectomycorrhizal types had identical PCR/RFLP profiles to corresponding fruit bodies from all investigated stands with no detectable intraspecific variation, despite the geographical distance of c.300 km between the sampling locations. By contrast, intraspecific variation between sporocarps from the different locations was detected in Paxillus rubicundulus, mycorrhizas of which were not found. The diversity of fruiting alder mycobionts at the main experimental plot only partly matched the diversity observed from mycorrhizas when comparing their PCR/RFLP profiles. The results are discussed regarding sampling techniques, PCR/RFLP analyses and ecological aspects 
650 4 |a Journal Article 
650 4 |a Alnus glutinosa 
650 4 |a Diversity 
650 4 |a ectomycorrhiza 
650 4 |a identification 
650 4 |a sporocarp 
700 1 |a Boyle, H  |e verfasserin  |4 aut 
700 1 |a Munch, J C  |e verfasserin  |4 aut 
700 1 |a Buscot, F  |e verfasserin  |4 aut 
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773 1 8 |g volume:137  |g year:1997  |g number:2  |g day:18  |g month:10  |g pages:357-369 
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