Transcriptomic analysis reveals the parallel transcriptional regulation of UV-B-induced artemisinin and flavonoid accumulation in Artemisia annua L

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 163(2021) vom: 01. Juni, Seite 189-200
1. Verfasser:	Li, Yongpeng (VerfasserIn)
Weitere Verfasser:	Qin, Wei, Fu, Xueqing, Zhang, Yaojie, Hassani, Danial, Kayani, Sadaf-Ilyas, Xie, Lihui, Liu, Hang, Chen, Tiantian, Yan, Xin, Peng, Bowen, Wu-Zhang, Kuanyu, Wang, Chen, Sun, Xiaofen, Li, Ling, Tang, Kexuan
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2021
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Artemisia annua Artemisinin Flavonoid accumulation Transcription regulation UV-B radiation Artemisinins Flavonoids

Beschreibung
Zusammenfassung:	Copyright © 2021 Elsevier Masson SAS. All rights reserved. UV-B radiation is a pivotal photomorphogenic signal and positively regulates plant growth and metabolite biosynthesis. In order to elucidate the transcriptional regulation mechanism underlying UV-B-induced artemisinin and flavonoid biosynthesis in Artemisia annua, the transcriptional responses of A. annua L. leaves to UV-B radiation were analyzed using the Illumina transcriptome sequencing. A total of 10705 differentially expressed genes (DEGs) including 533 transcription factors (TFs), were identified. Based on the expression trends of the differentially expressed TFs as well as artemisinin and flavonoid biosynthesis genes, we speculated that TFs belonging to 6 clusters were most likely to be involved in the regulation of artemisinin and/or flavonoid biosynthesis. The regulatory relationship between TFs and artemisinin/flavonoid biosynthetic genes was further studied. Dual-LUC assays results showed that AaMYB6 is a positive regulator of AaLDOX which belongs to flavonoid biosynthesis pathway. In addition, we identified an R2R3 MYB TF, AaMYB4 which potentially mediated both artemisinin and flavonoid biosynthesis pathways by activating the expression of AaADS and AaDBR2 in artemisinin biosynthesis pathway and AaUFGT in flavonoid biosynthesis pathway. Overall, our findings would provide an insight into the elucidation of the parallel transcriptional regulation of artemisinin and flavonoid biosynthesis in A. annua L. under UV-B radiation
Beschreibung:	Date Completed 12.05.2021 Date Revised 12.05.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2021.03.052