Suppression of an elicitor-induced oxidative burst reaction in Medicago sativa cell cultures by Sinorhizobium meliloti lipopolysaccharides

•  The biological activity of lipopolysaccharides (LPS) from the symbiotic soil bacterium Sinorhizobium meliloti was analysed in cell cultures of the host plant Medicago sativa (alfalfa) and the nonhost plant Nicotiana tabacum (tobacco). •  LPS of S. meliloti were purified and chemically characteriz...

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Veröffentlicht in:The New phytologist. - 1979. - 151(2001), 3 vom: 28. Sept., Seite 597-606
1. Verfasser: Albus, Ulrike (VerfasserIn)
Weitere Verfasser: Baier, Ruth, Holst, Otto, Pühler, Alfred, Niehaus, Karsten
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Medicago sativa Nicotiana tabacum (tobacco) Sinorhizobium meliloti lipopolysaccharides oxidative burst plant defence symbiosis
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520 |a •  The biological activity of lipopolysaccharides (LPS) from the symbiotic soil bacterium Sinorhizobium meliloti was analysed in cell cultures of the host plant Medicago sativa (alfalfa) and the nonhost plant Nicotiana tabacum (tobacco). •  LPS of S. meliloti were purified and chemically characterized. Alfalfa and tobacco suspension cell cultures responded to yeast elicitors with an alkalinization of the culture medium and the induction of an oxidative burst. This assay was used to study the biological activity of isolated LPS. •  In alfalfa cell cultures the simultaneous addition of purified LPS of S. meliloti suppressed the elicitor induced alkalinization and oxidative burst reaction. Cell cultures of the nonhost tobacco reacted differently to the application of S. meliloti LPS. In these cell cultures, the S. meliloti LPS itself caused an alkalinization of the culture medium and an oxidative burst reaction. •  S. meliloti LPS released from the bacterial surface might function as a specific signal molecule, promoting the symbiotic interaction and suppressing a pathogenic response in the host plant, alfalfa 
650 4 |a Journal Article 
650 4 |a Medicago sativa 
650 4 |a Nicotiana tabacum (tobacco) 
650 4 |a Sinorhizobium meliloti 
650 4 |a lipopolysaccharides 
650 4 |a oxidative burst 
650 4 |a plant defence 
650 4 |a symbiosis 
700 1 |a Baier, Ruth  |e verfasserin  |4 aut 
700 1 |a Holst, Otto  |e verfasserin  |4 aut 
700 1 |a Pühler, Alfred  |e verfasserin  |4 aut 
700 1 |a Niehaus, Karsten  |e verfasserin  |4 aut 
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