Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey
© 2020 Authors.
| Veröffentlicht in: | Journal of nematology. - 1969. - 52(2020) vom: 02. |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | Journal of nematology |
| Schlagworte: | Journal Article Barley D2-D3 expansion region of 28S rRNA Detection Real-time PCR Sequencing Walnut root lesion nematode Wheat |
| Zusammenfassung: | © 2020 Authors. Pratylenchus vulnus (walnut root lesion nematode) is one of the most damaging root lesion nematode species worldwide. In this study, 17 populations of P. vulnus obtained from wheat and barley cultivated fields in 2016 to 2017 in Turkey (Karaman and Konya provinces) were identified using real-time PCR and melting curve analysis. Samples provided a single peak at 87.3˚C with real-time PCR. D2 to D3 expansion segments of the 28S rRNA of one population from Cihanbeyli district in Konya province was sequenced and recorded in GenBank (Accession number: MT320536.1). Alignments of the population was identical 98.66% to the populations of P. vulnus available in GenBank (Accs. nos: LT985479.1 and LT965052.1) and 98.65% (Accs. nos: KY424305.1 and KY424304.1) |
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| Beschreibung: | Date Revised 09.04.2021 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
| ISSN: | 0022-300X |
| DOI: | 10.21307/jofnem-2020-084 |