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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-021-02395-5
|2 doi
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|a pubmed24n1077.xml
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|a (DE-627)NLM323262228
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|a (NLM)33770306
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Diehl, Otávio J
|e verfasserin
|4 aut
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|a Antenna regeneration as an ecotoxicological endpoint in a marine amphipod
|b a proof of concept using dimethyl sulfoxide and diflubenzuron
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 23.04.2021
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|a Date Revised 23.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Regeneration is a widely spread process across the animal kingdom, including many species of marine crustaceans. It is strongly linked to hormonal cycles and, therefore, a great endpoint candidate for toxicology studies. We selected the amphipod Parhyale hawaiensis as test organism, already used in ecotoxicological studies and able to regenerate its body appendages. We are proposing a protocol to use the antenna regeneration as a toxicity endpoint. First, we evaluated differences in time of completion of regeneration in males and females after the amputation of one antenna of 6 months old animals. Then we compared the influence of different testing volumes in the regeneration process (100 and 5 mL). We used as testing substances, dimethyl sulfoxide (DMSO) and diflubenzuron, a chitin synthesis inhibitor. The most suitable protocol consisted of volumes of 5 mL in 12-well microplates, with 1 organism per well, 12 organisms per concentration (1:1 females/males) and test time duration of around 5 weeks. DMSO accelerated regeneration time with a NOEC of 0.06%. Diflubenzuron inhibited the time necessary to its completion with a NOEC of 0.32 μg L-1. We conclude that the Parhyale hawaiensis antenna regeneration protocol proposed here is a potential tool in ecotoxicology, but more studies are required for its validation not only to verify its utility for testing chemicals but also environmental samples
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|a Journal Article
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|a DMSO
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|a Development impairment
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|a Growth inhibitor
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|a Parhyale hawaiensis
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|a Pesticide
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|a Regeneration
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|a Diflubenzuron
|2 NLM
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|a J76U6ZSI8D
|2 NLM
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|a Dimethyl Sulfoxide
|2 NLM
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| 650 |
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|a YOW8V9698H
|2 NLM
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| 700 |
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|a Assano, Patrícia K
|e verfasserin
|4 aut
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|a da Costa, Thiago Roncini G
|e verfasserin
|4 aut
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| 700 |
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|a Oliveira, Rhaul
|e verfasserin
|4 aut
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|a Marques-Souza, Henrique
|e verfasserin
|4 aut
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|a Umbuzeiro, Gisela de A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 30(2021), 4 vom: 01. Mai, Seite 751-755
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnns
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|g volume:30
|g year:2021
|g number:4
|g day:01
|g month:05
|g pages:751-755
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|u http://dx.doi.org/10.1007/s10646-021-02395-5
|3 Volltext
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