Valorization of pulp and paper industry wastewater using sludge enriched with nitrogen-fixing bacteria
© 2021 The Authors. Water Environment Research published by Wiley Periodicals LLC on behalf of Water Environment Federation.
| Veröffentlicht in: | Water environment research : a research publication of the Water Environment Federation. - 1998. - 93(2021), 9 vom: 23. Sept., Seite 1734-1747 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | Water environment research : a research publication of the Water Environment Federation |
| Schlagworte: | Journal Article biofertilizer biological nitrogen fixation biological treatment high C:N wastewater treatment nitrogen-fixing bacteria pulp and paper mill effluent Industrial Waste RNA, Ribosomal, 16S Sewage mehr... |
| Zusammenfassung: | © 2021 The Authors. Water Environment Research published by Wiley Periodicals LLC on behalf of Water Environment Federation. Nitrogen-fixing bacteria (NFB) can reduce nitrogen at ambient pressure and temperature. In this study, we treated effluent from a paper mill in sequencing batch reactors (SBRs) and monitored the abundance and activity of NFB with a view to producing a sludge that could work as a biofertilizer. Four reactors were inoculated with activated sludge enriched with NFB and fed with a high C/N waste (100:0.5) from a paper mill. Though the reactors were able to reduce the organic load of the wastewater by up to 89%, they did not have any nitrogen-fixing activity and showed a decrease in the putative number of NFB (quantified with qPCR). The most abundant species in the reactors treating high C/N paper mill wastewater was identified by Illumina MiSeq 16S rRNA gene amplicon sequencing as Methyloversatilis sp. (relative abundance of 4.4%). Nitrogen fixation was observed when the C/N ratio was increased by adding sucrose. We suspect that real-world biological nitrogen fixation (BNF) will only occur where there is a C/N ratio ≤100:0.07. Consequently, operators should actively avoid adding or allowing nitrogen in the waste streams if they wish to valorize their sludge and reduce running costs. PRACTITIONER POINTS: Efficient biological wastewater treatment of low nitrogen paper mill effluent was achieved without nutrient supplementation. The sludge was still capable of fixing nitrogen although this process was not observed in the wastewater treatment system. This high C/N wastewater treatment technology could be used with effluents from cassava flour, olive oil, wine and dairy industries |
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| Beschreibung: | Date Completed 08.09.2021 Date Revised 07.12.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1554-7531 |
| DOI: | 10.1002/wer.1561 |