Cis-regulation of the amino acid transporter genes ZmAAP2 and ZmLHT1 by ZmPHR1 transcription factors in maize ear under phosphate limitation

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 72(2021), 10 vom: 04. Mai, Seite 3846-3863
1. Verfasser:	Wang, Ruifeng (VerfasserIn)
Weitere Verfasser:	Zhong, Yanting, Liu, Xiaoting, Zhao, Cheng, Zhao, Jianyu, Li, Mengfei, Ul Hassan, Mahmood, Yang, Bo, Li, Dongdong, Liu, Renyi, Li, Xuexian
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2021
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't ZmAAP2 ZmLHT1 ZmPHR1s Amino acid transporter ear maize phosphate Amino Acid Transport Systems mehr... Phosphates Transcription Factors

Beschreibung
Zusammenfassung:	© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Phosphorus and nitrogen nutrition have profound and complicated innate connections; however, underlying molecular mechanisms are mostly elusive. PHR1 is a master phosphate signaling component, and whether it directly functions in phosphorus-nitrogen crosstalk remains a particularly interesting question. In maize, nitrogen limitation caused tip kernel abortion and ear shortening. By contrast, moderately low phosphate in the field reduced kernels across the ear, maintained ear elongation and significantly lowered concentrations of total free amino acids and soluble proteins 2 weeks after silking. Transcriptome profiling revealed significant enrichment and overall down-regulation of transport genes in ears under low phosphate. Importantly, 313 out of 847 differentially expressed genes harbored PHR1 binding sequences (P1BS) including those controlling amino acid/polyamine transport and metabolism. Specifically, both ZmAAP2 and ZmLHT1 are plasma membrane-localized broad-spectrum amino acid transporters, and ZmPHR1.1 and ZmPHR1.2 were able to bind to P1BS-containing ZmAAP2 and ZmLHT1 and down-regulate their expression in planta. Taken together, the results suggest that prevalence of P1BS elements enables ZmPHR1s to regulate a large number of low phosphate responsive genes. Further, consistent with reduced accumulation of free amino acids, ZmPHR1s down-regulate ZmAAP2 and ZmLHT1 expression as direct linkers of phosphorus and nitrogen nutrition independent of NIGT1 in maize ear under low phosphate
Beschreibung:	Date Completed 21.05.2021 Date Revised 21.05.2021 published: Print Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erab103