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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202008451
|2 doi
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|a pubmed24n1566.xml
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Maihöfer, Johanna
|e verfasserin
|4 aut
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| 245 |
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|a Hydrogel-Guided, rAAV-Mediated IGF-I Overexpression Enables Long-Term Cartilage Repair and Protection against Perifocal Osteoarthritis in a Large-Animal Full-Thickness Chondral Defect Model at One Year In Vivo
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 24.07.2024
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|a Date Revised 13.10.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a The regeneration of focal articular cartilage defects is complicated by the reduced quality of the repair tissue and the potential development of perifocal osteoarthritis (OA). Biomaterial-guided gene therapy may enhance cartilage repair by controlling the release of therapeutic sequences in a spatiotemporal manner. Here, the benefits of delivering a recombinant adeno-associated virus (rAAV) vector coding for the human insulin-like growth factor I (IGF-I) via an alginate hydrogel (IGF-I/AlgPH155) to enhance repair of full-thickness chondral defects following microfracture surgery after one year in minipigs versus control (lacZ/AlgPH155) treatment are reported. Sustained IGF-I overexpression is significantly achieved in the repair tissue of defects treated with IGF-I/AlgPH155 versus those receiving lacZ/AlgPH155 for one year and in the cartilage surrounding the defects. Administration of IGF-I/AlgPH155 significantly improves parameters of cartilage repair at one year relative to lacZ/AlgPH155 (semiquantitative total histological score, cell densities, matrix deposition) without deleterious or immune reactions. Remarkably, delivery of IGF-I/AlgPH155 also significantly reduces perifocal OA and inflammation after one year versus lacZ/AlgPH155 treatment. Biomaterial-guided rAAV gene transfer represents a valuable clinical approach to promote cartilage repair and to protect against OA
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|a Journal Article
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|a IGF-I
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|a alginate hydrogel
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|a cartilage repair
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|a perifocal osteoarthritis
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|a rAAV
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|a Insulin-Like Growth Factor I
|2 NLM
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| 650 |
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|a 67763-96-6
|2 NLM
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| 650 |
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|a Hydrogels
|2 NLM
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| 650 |
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|a Alginates
|2 NLM
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| 700 |
1 |
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|a Madry, Henning
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rey-Rico, Ana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Venkatesan, Jagadeesh K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Goebel, Lars
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schmitt, Gertrud
|e verfasserin
|4 aut
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1 |
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|a Speicher-Mentges, Susanne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cai, Xiaoyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Meng, Weikun
|e verfasserin
|4 aut
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| 700 |
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|a Zurakowski, David
|e verfasserin
|4 aut
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| 700 |
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|a Menger, Michael D
|e verfasserin
|4 aut
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| 700 |
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|a Laschke, Matthias W
|e verfasserin
|4 aut
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| 700 |
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|a Cucchiarini, Magali
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 33(2021), 16 vom: 04. Apr., Seite e2008451
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
1 |
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|g volume:33
|g year:2021
|g number:16
|g day:04
|g month:04
|g pages:e2008451
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202008451
|3 Volltext
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|d 33
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