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20231225182923.0 |
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cr uuu---uuuuu |
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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-021-02367-9
|2 doi
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|a pubmed24n1076.xml
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|a (DE-627)NLM322851513
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|a (NLM)33728520
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Tang, Dan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Toxic effects of metal copper stress on immunity, metabolism and pathologic changes in Chinese mitten crab (Eriocheir japonica sinensis)
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 23.04.2021
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|a Date Revised 23.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copper (Cu2+), which represents a major physiological challenge for crab culture, is ubiquitous in the aquatic culture environment, and gills are the first organs that come into direct contact with the environment. However, the molecular basis of the response of crabs to Cu2+ stress remains unclear. Here, we conducted a transcriptome and differential expression analysis on the gills from Chinese mitten crab unexposed and exposed to Cu2+ for 24 h. The comparative transcriptome analysis identified 2486 differentially expressed genes (DEGs). GO functional analysis and KEGG pathway analysis revealed some DEGs, which were mostly related to immunity, metabolism, osmotic regulation, Cu2+ homeostasis regulation, antioxidant activity, and detoxification process. Some pathways related to humoral and cellular immunity, such as phagosome, peroxisome, lysosome, mTOR signaling pathway, PI3K-Akt signaling pathway, Toll-like receptor signaling pathway, and T cell receptor signaling pathway were enhanced under Cu2+ stress. In addition, Cu2+ stress altered the expression patterns of key phagocytosis and apoptosis genes (lectin, cathepsin L, Rab7, and HSP70), confirming that Cu2+ can induce oxidative stress and eventually even apoptosis. Histological analysis revealed that the copper can induce damage at the cellular level. This comparative transcriptome analysis provides valuable molecular information to aid future study of the immune mechanism of Chinese mitten crab in response to Cu2+ stress and provides a foundation for further understanding of the effects of metal toxicity
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|a Journal Article
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|a Chinese mitten crab
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4 |
|a Cu2+ toxicity
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| 650 |
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4 |
|a Detoxification process
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| 650 |
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4 |
|a Immunity
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| 650 |
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4 |
|a Transcriptome
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| 650 |
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7 |
|a Copper
|2 NLM
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| 650 |
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7 |
|a 789U1901C5
|2 NLM
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| 700 |
1 |
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|a Liu, Ruobing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shi, Xueling
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shen, Chenchen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bai, Yuze
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Boping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Zhengfei
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 30(2021), 4 vom: 16. Mai, Seite 632-642
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnns
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| 773 |
1 |
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|g volume:30
|g year:2021
|g number:4
|g day:16
|g month:05
|g pages:632-642
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|u http://dx.doi.org/10.1007/s10646-021-02367-9
|3 Volltext
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