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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erab116
|2 doi
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|a pubmed25n1075.xml
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|a (DE-627)NLM32267946X
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|a (NLM)33711094
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Ma, Dawei
|e verfasserin
|4 aut
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|a Poplar MYB117 promotes anthocyanin synthesis and enhances flavonoid B-ring hydroxylation by up-regulating the flavonoid 3',5'-hydroxylase gene
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 21.05.2021
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|a Date Revised 21.05.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Flavonoids, such as anthocyanins, proanthocyanidins, and flavonols, are widespread plant secondary metabolites and important for plant adaptation to diverse abiotic and biotic stresses. Flavonoids can be variously hydroxylated and decorated; their biological activity is partly dependent on the degree of hydroxylation of the B-ring. Flavonoid biosynthesis is regulated by MYB transcription factors, which have been identified and characterized in a diversity of plants. Here we characterize a new MYB activator, MYB117, in hybrid poplar (Populus tremula×tremuloides). When overexpressed in transgenic poplar plants, MYB117 enhanced anthocyanin accumulation in all tissues. Transcriptome analysis of MYB117-overexpressing poplars confirmed the up-regulation of flavonoid and anthocyanin biosynthesis genes, as well as two flavonoid 3',5'-hydroxylase (F3'5'H) genes. We also identified up-regulated cytochrome b5 genes, required for full activity of F3'5'H . Phytochemical analysis demonstrated a corresponding increase in B-ring hydroxylation of anthocyanins, proanthocyanidins, and flavonols in these transgenics. Similarly, overexpression of F3'5'H1 directly in hybrid poplar also resulted in increased B-ring hydroxylation, but without affecting overall flavonoid content. However, the overexpression of the cytochrome b5 gene in F3'5'H1-overexpressing plants did not further increase B-ring hydroxylation. Our data indicate that MYB117 regulates the biosynthesis of anthocyanins in poplar, but also enhances B-ring hydroxylation by up-regulating F3'5'H1
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Anthocyanin
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|a MYB
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|a RNA-seq
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|a cytochrome b5
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|a flavonoid
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|a flavonoid 3′,5′-hydroxylase
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|a Anthocyanins
|2 NLM
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| 650 |
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|a Flavonoids
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Cytochrome P-450 Enzyme System
|2 NLM
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| 650 |
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|a 9035-51-2
|2 NLM
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| 650 |
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|a flavonoid 3',5'-hydroxylase
|2 NLM
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| 650 |
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|a EC 1.14.-
|2 NLM
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| 700 |
1 |
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|a Tang, Hao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Reichelt, Michael
|e verfasserin
|4 aut
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| 700 |
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|a Piirtola, Eerik-Mikael
|e verfasserin
|4 aut
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| 700 |
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|a Salminen, Juha-Pekka
|e verfasserin
|4 aut
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|a Gershenzon, Jonathan
|e verfasserin
|4 aut
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| 700 |
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|a Constabel, C Peter
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 72(2021), 10 vom: 04. Mai, Seite 3864-3880
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
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|g volume:72
|g year:2021
|g number:10
|g day:04
|g month:05
|g pages:3864-3880
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|u http://dx.doi.org/10.1093/jxb/erab116
|3 Volltext
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|d 72
|j 2021
|e 10
|b 04
|c 05
|h 3864-3880
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