Metabolomics-driven gene mining and genetic improvement of tolerance to salt-induced osmotic stress in maize

Metabolomics-driven gene mining and genetic improvement of tolerance to salt-induced osmotic stress in maize

© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 230(2021), 6 vom: 30. Juni, Seite 2355-2370
1. Verfasser: Liang, Xiaoyan (VerfasserIn)
Weitere Verfasser: Liu, Songyu, Wang, Tao, Li, Fenrong, Cheng, Jinkui, Lai, Jinsheng, Qin, Feng, Li, Zhen, Wang, Xiangfeng, Jiang, Caifu
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't genetic improvement mGWAS maize metabolomics profiling salt tolerance
Beschreibung
Zusammenfassung:© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.
The farmland of the world's main corn-producing area is increasingly affected by salt stress. Therefore, the breeding of salt-tolerant cultivars is necessary for the long-term sustainability of global corn production. Previous studies have shown that natural maize varieties display a large diversity of salt tolerance, yet the genetic variants underlying such diversity remain poorly discovered and applied, especially those mediating the tolerance to salt-induced osmotic stress (SIOS). Here we report a metabolomics-driven understanding and genetic improvement of maize SIOS tolerance. Using a LC-MS-based untargeted metabolomics approach, we profiled the metabolomes of 266 maize inbred lines under control and salt conditions, and then identified 37 metabolite biomarkers of SIOS tolerance (METO1-37). Follow-up metabolic GWAS (mGWAS) and genotype-to-phenotype modeling identified 10 candidate genes significantly associating with the SIOS tolerance and METO abundances. Furthermore, we validated that a citrate synthase, a glucosyltransferase and a cytochrome P450 underlie the genotype-METO-SIOS tolerance associations, and showed that their favorable alleles additively improve the SIOS tolerance of elite maize inbred lines. Our study provides a novel insight into the natural variation of maize SIOS tolerance, which boosts the genetic improvement of maize salt tolerance, and demonstrates a metabolomics-based approach for mining crop genes associated with this complex agronomic trait
Beschreibung:Date Completed 04.06.2021
Date Revised 04.06.2021
published: Print-Electronic
CommentIn: New Phytol. 2021 Jun;230(6):2091-2093. - PMID 33880794
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.17323