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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-11-20-2439-RE
|2 doi
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|a eng
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|a Kaur, Harleen
|e verfasserin
|4 aut
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|a A Diagnostic TaqMan Real-Time PCR Assay for In Planta Detection and Quantification of Colletotrichum theobromicola, Causal Agent of Boxwood Dieback
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 10.12.2021
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|a Date Revised 14.12.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Boxwood dieback, caused by Colletotrichum theobromicola, is spreading at an alarming rate in the boxwood industry in the United States. Although C. theobromicola has been accepted as a distinct species within the C. gloeosporioides species complex, it is difficult to distinguish it from other closely related species based on morphology. Moreover, molecular identification of C. theobromicola requires amplification and sequencing of multiple loci, which can be expensive and time consuming. Therefore, a diagnostic TaqMan real-time PCR assay was developed for early and accurate detection and quantification of C. theobromicola in boxwood. The study involved the design of species-specific primers and a TaqMan probe to differentiate C. theobromicola from other closely related Colletotrichum species. The primers and probe discriminate between C. theobromicola and other species in the C. gloeosporioides species complex and can detect C. theobromicola at very low concentrations, illustrating the high specificity and sensitivity of the assay. This TaqMan real-time PCR assay accurately and rapidly distinguishes boxwood dieback from other diseases with similar symptomatology, including Macrophoma blight, Phytophthora root rot, and Volutella blight, as well as some disorders produced by abiotic agents
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|a Journal Article
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|a Buxus spp.
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|a Colletotrichum theobromicola
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|a TaqMan
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|a boxwood
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|a diagnostic assay
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| 650 |
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|a dieback
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| 650 |
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|a probe
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| 650 |
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|a real-time PCR
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| 650 |
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4 |
|a species-specific primers
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| 700 |
1 |
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|a Singh, Raghuwinder
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Doyle, Vinson
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Valverde, Rodrigo
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 105(2021), 9 vom: 25. Sept., Seite 2395-2401
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnas
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| 773 |
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|g volume:105
|g year:2021
|g number:9
|g day:25
|g month:09
|g pages:2395-2401
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|u http://dx.doi.org/10.1094/PDIS-11-20-2439-RE
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