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|a 10.1111/nph.17292
|2 doi
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|a pubmed24n1072.xml
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|e rakwb
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|a eng
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|a Jiquel, Audren
|e verfasserin
|4 aut
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|a A gene-for-gene interaction involving a 'late' effector contributes to quantitative resistance to the stem canker disease in Brassica napus
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 21.07.2021
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|a Date Revised 31.05.2022
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|a published: Print-Electronic
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|a CommentIn: New Phytol. 2021 Aug;231(4):1301-1303. - PMID 34107082
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|a Citation Status MEDLINE
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|a © 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.
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|a The control of stem canker disease of Brassica napus (rapeseed), caused by the fungus Leptosphaeria maculans is based largely on plant genetic resistance: single-gene specific resistance (Rlm genes) or quantitative, polygenic, adult-stage resistance. Our working hypothesis was that quantitative resistance partly obeys the gene-for-gene model, with resistance genes 'recognizing' fungal effectors expressed during late systemic colonization. Five LmSTEE (stem-expressed effector) genes were selected and placed under the control of the AvrLm4-7 promoter, an effector gene highly expressed at the cotyledon stage of infection, for miniaturized cotyledon inoculation test screening of a gene pool of 204 rapeseed genotypes. We identified a rapeseed genotype, 'Yudal', expressing hypersensitive response to LmSTEE98. The LmSTEE98-RlmSTEE98 interaction was further validated by inactivation of the LmSTEE98 gene with a CRISPR-Cas9 approach. Isolates with mutated versions of LmSTEE98 induced more severe stem symptoms than the wild-type isolate in 'Yudal'. This single-gene resistance was mapped in a 0.6 cM interval of the 'Darmor_bzh' × 'Yudal' genetic map. One typical gene-for-gene interaction contributes partly to quantitative resistance when L. maculans colonizes the stems of rapeseed. With numerous other effectors specific to stem colonization, our study provides a new route for resistance gene discovery, elucidation of quantitative resistance mechanisms and selection for durable resistance
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Brassica napus
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|a Leptosphaeria maculans
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|a effector
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|a gene-for-gene interaction
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|a quantitative disease resistance
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|a Gervais, Julie
|e verfasserin
|4 aut
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| 700 |
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|a Geistodt-Kiener, Aude
|e verfasserin
|4 aut
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| 700 |
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|a Delourme, Régine
|e verfasserin
|4 aut
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| 700 |
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|a Gay, Elise J
|e verfasserin
|4 aut
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| 700 |
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|a Ollivier, Bénédicte
|e verfasserin
|4 aut
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| 700 |
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|a Fudal, Isabelle
|e verfasserin
|4 aut
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| 700 |
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|a Faure, Sébastien
|e verfasserin
|4 aut
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| 700 |
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|a Balesdent, Marie-Hélène
|e verfasserin
|4 aut
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| 700 |
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|a Rouxel, Thierry
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 231(2021), 4 vom: 15. Aug., Seite 1510-1524
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|x 1469-8137
|7 nnns
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| 773 |
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|g volume:231
|g year:2021
|g number:4
|g day:15
|g month:08
|g pages:1510-1524
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|u http://dx.doi.org/10.1111/nph.17292
|3 Volltext
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