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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/nph.17272
|2 doi
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|a pubmed24n1071.xml
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|a (DE-627)NLM321370023
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|a (NLM)33577135
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Dindas, Julian
|e verfasserin
|4 aut
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|a A voltage-dependent Ca2+ homeostat operates in the plant vacuolar membrane
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 14.05.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a ©2021 The Authors New Phytologist © 2021 New Phytologist Foundation.
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|a Cytosolic calcium signals are evoked by a large variety of biotic and abiotic stimuli and play an important role in cellular and long distance signalling in plants. While the function of the plasma membrane in cytosolic Ca2+ signalling has been intensively studied, the role of the vacuolar membrane remains elusive. A newly developed vacuolar voltage clamp technique was used in combination with live-cell imaging, to study the role of the vacuolar membrane in Ca2+ and pH homeostasis of bulging root hair cells of Arabidopsis. Depolarisation of the vacuolar membrane caused a rapid increase in the Ca2+ concentration and alkalised the cytosol, while hyperpolarisation led to the opposite responses. The relationship between the vacuolar membrane potential, the cytosolic pH and Ca2+ concentration suggests that a vacuolar H+ /Ca2+ exchange mechanism plays a central role in cytosolic Ca2+ homeostasis. Mathematical modelling further suggests that the voltage-dependent vacuolar Ca2+ homeostat could contribute to calcium signalling when coupled to a recently discovered K+ channel-dependent module for electrical excitability of the vacuolar membrane
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis thaliana
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|a R-GECO1 cytosolic Ca2+ reporter
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|a TPC1 channel
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|a calcium signalling
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|a computational cell biology
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|a cpYFP cytosolic pH reporter
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|a vacuolar membrane
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|a voltage clamp
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|a Arabidopsis Proteins
|2 NLM
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|a Calcium Channels
|2 NLM
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|a Calcium
|2 NLM
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|a SY7Q814VUP
|2 NLM
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| 700 |
1 |
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|a Dreyer, Ingo
|e verfasserin
|4 aut
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| 700 |
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|a Huang, Shouguang
|e verfasserin
|4 aut
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| 700 |
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|a Hedrich, Rainer
|e verfasserin
|4 aut
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| 700 |
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|a Roelfsema, M Rob G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 230(2021), 4 vom: 12. Mai, Seite 1449-1460
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:230
|g year:2021
|g number:4
|g day:12
|g month:05
|g pages:1449-1460
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|u http://dx.doi.org/10.1111/nph.17272
|3 Volltext
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