How to easily detect plant NADH-glutamate dehydrogenase (GDH) activity? A simple and reliable in planta procedure suitable for tissues, extracts and heterologous microbial systems
Copyright © 2020 Elsevier B.V. All rights reserved.
| Publié dans: | Plant science : an international journal of experimental plant biology. - 1985. - 304(2021) vom: 20. März, Seite 110714 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2021
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| Accès à la collection: | Plant science : an international journal of experimental plant biology |
| Sujets: | Journal Article Arabidopsis thaliana mutants GDH GDH rapid colorimetric method In planta enzymatic assay Plant glutamate dehydrogenase Zymogram Coloring Agents Plant Extracts Glutamate Dehydrogenase |
| Résumé: | Copyright © 2020 Elsevier B.V. All rights reserved. Plant NADH glutamate dehydrogenase (GDH) is an intriguing enzyme, since it is involved in different metabolic processes owing to its reversible (anabolic/catabolic) activity and due to the oligomeric nature of the enzyme, that gives rise to several isoforms. The complexity of GDH isoenzymes pattern and the variability of the spatial and temporal localization of the different isoforms have limited our comprehension of the physiological role of GDH in plants. Genetics, immunological, and biochemical approaches have been used until now in order to shed light on the regulatory mechanism that control GDH expression in different plant systems and environmental conditions. We describe here the validation of a simple in planta GDH activity staining procedure, providing evidence that it might be used, with different purposes, to determine GDH expression in plant organs, tissues, extracts and also heterologous systems |
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| Description: | Date Completed 17.05.2021 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2020.110714 |