Role of type I NADH dehydrogenase in Synechocystis sp. PCC 6803 under phycobilisome excited red light
Copyright © 2020 Elsevier B.V. All rights reserved.
| Publié dans: | Plant science : an international journal of experimental plant biology. - 1985. - 304(2021) vom: 20. März, Seite 110798 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2021
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| Accès à la collection: | Plant science : an international journal of experimental plant biology |
| Sujets: | Journal Article Cyanobacteria Flux balance analysis Genome-scale model NDH-1 PSI/PSII excitation ratio Bacterial Proteins Photosystem I Protein Complex Photosystem II Protein Complex Phycobilisomes plus... |
| Résumé: | Copyright © 2020 Elsevier B.V. All rights reserved. Cyanobacterial type I NADH dehydrogenase (NDH-1) is involved in various bioenergetic reactions including respiration, cyclic electron transport (CET), and CO2 uptake. The role of NDH-1 is usually minor under normal growth conditions and becomes important under stress conditions. However, in our previous study, flux balance analysis (FBA) simulation predicted that the drive of NDH-1 as CET pathway with a photosystem (PS) I/PSII excitation ratio around 1.0 contributes to achieving an optimal specific growth rate. In this study, to experimentally elucidate the predicted functions of NDH-1, first, we measured the PSI/PSII excitation ratios of Synechocystis sp. PCC 6803 grown under four types of spectral light conditions. The specific growth rate was the highest and PSI/PSII excitation ratio was with 0.88 under the single-peak light at 630 nm (Red1). Considering this measured excitation ratios, FBA simulation predicted that NDH-1-dependent electron transport was the major pathway under Red1. Moreover, in actual culture, an NDH-1 deletion strain had slower growth rate than that of wild type only under Red1 light condition. Therefore, we experimentally demonstrated that NDH-1 plays an important role under optimal light conditions such as Red1 light, where Synechocystis exhibits the highest specific growth rate and PSI/PSII excitation ratio of around 1.0 |
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| Description: | Date Completed 17.05.2021 Date Revised 17.05.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2020.110798 |