VviNAC33 promotes organ de-greening and represses vegetative growth during the vegetative-to-mature phase transition in grapevine

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 231(2021), 2 vom: 10. Juli, Seite 726-746
1. Verfasser:	D'Incà, Erica (VerfasserIn)
Weitere Verfasser:	Cazzaniga, Stefano, Foresti, Chiara, Vitulo, Nicola, Bertini, Edoardo, Galli, Mary, Gallavotti, Andrea, Pezzotti, Mario, Battista Tornielli, Giovanni, Zenoni, Sara
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2021
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. DAP-seq NAC33 de-greening grapevine phase transition transcriptomics vegetative growth

Beschreibung
Zusammenfassung:	© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation. Plants undergo several developmental transitions during their life cycle. In grapevine, a perennial woody fruit crop, the transition from vegetative/green-to-mature/woody growth involves transcriptomic reprogramming orchestrated by a small group of genes encoding regulators, but the underlying molecular mechanisms are not fully understood. We investigated the function of the transcriptional regulator VviNAC33 by generating and characterizing transgenic overexpressing grapevine lines and a chimeric repressor, and by exploring its putative targets through a DNA affinity purification sequencing (DAP-seq) approach combined with transcriptomic data. We demonstrated that VviNAC33 induces leaf de-greening, inhibits organ growth and directly activates the expression of STAY-GREEN PROTEIN 1 (SGR1), which is involved in Chl and photosystem degradation, and AUTOPHAGY 8f (ATG8f), which is involved in the maturation of autophagosomes. Furthermore, we show that VviNAC33 directly inhibits AUXIN EFFLUX FACILITATOR PIN1, RopGEF1 and ATP SYNTHASE GAMMA CHAIN 1T (ATPC1), which are involved in photosystem II integrity and activity. Our results show that VviNAC33 plays a major role in terminating photosynthetic activity and organ growth as part of a regulatory network governing the vegetative-to-mature phase transition
Beschreibung:	Date Completed 18.06.2021 Date Revised 07.07.2021 published: Print-Electronic CommentIn: New Phytol. 2021 Jul;231(2):505-507. - PMID 34132413 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.17263