Peach PpSnRK1α interacts with bZIP11 and maintains trehalose balance in plants
Copyright © 2021 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 160(2021) vom: 01. März, Seite 377-385 |
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| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Peach Plant growth SnRK1 Trehalose bZIP11 Basic-Leucine Zipper Transcription Factors Sugar Phosphates B8WCK70T7I Protein Serine-Threonine Kinases |
| Zusammenfassung: | Copyright © 2021 The Author(s). Published by Elsevier Masson SAS.. All rights reserved. The nonreducing disaccharide trehalose is widespread in nature. It plays a very important role in plant growth and development. In plants, trehalose is present in trace amounts. High concentration of trehalose disrupts energy balance and inhibits normal growth and development. Studies have shown that high levels of trehalose and trehalose-6-phosphate (T6P), the metabolic precursor of trehalose, inhibit sucrose non-fermenting-1-related protein kinase1 (SnRK1) activity, which affect plant growth and development. However, the role of SnRK1, the energy balance center, in the regulation of trehalose metabolism in plants is unknown. In this study, exogenous trehalose at higher concentrations inhibited the expression of SnRK1 genes, especially PpSnRK1α in peach (Prunus persica) seedlings. This change in gene expression was dependent on trehalose concentration. Furthermore, overexpression of peach PpSnRK1α in Arabidopsis thaliana significantly promoted trehalase activity, reduced T6P content, and suppressed the trehalose synthesis related genes (TPSs, TPPB) expression, promoted the trehalose metabolism of gene expression (TRE1), in addition the transgenic plants alleviated photosynthetic product distribution imbalance (aboveground and underground parts), and enhanced root growth. Yeast two-hybrid and bimolecular fluorescence assays revealed the interaction between PpSnRK1α and peach basic domain leucine zipper transcription factor 11 (PpbZIP11), a key transcription factor of trehalose metabolism, in the nucleus. To summarize, PpSnRK1α overexpression improved bZIP11 transcriptional activity and regulated trehalose metabolism to protect the plants against trehalose-induced damage. This study preliminarily explained the mechanism of SnRK1 regulating trehalose metabolism balance in plants, which laid a foundation for further understanding of energy metabolism and function of SnRK1 in plants |
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| Beschreibung: | Date Completed 02.03.2021 Date Revised 04.12.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2021.01.036 |