The microRNA476a-RFL module regulates adventitious root formation through a mitochondria-dependent pathway in Populus

The microRNA476a-RFL module regulates adventitious root formation through a mitochondria-dependent pathway in Populus

© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 230(2021), 5 vom: 03. Juni, Seite 2011-2028
1. Verfasser: Xu, Changzheng (VerfasserIn)
Weitere Verfasser: Tao, Yuanxun, Fu, Xiaokang, Guo, Li, Xing, Haitao, Li, Chaofeng, Yang, Ziwei, Su, Huili, Wang, Xianqiang, Hu, Jian, Fan, Di, Chiang, Vincent L, Luo, Keming
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Populus adventitious root auxin miR476a mitochondrion pentatricopeptide repeat protein Indoleacetic Acids
Beschreibung
Zusammenfassung:© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.
For woody plants, clonal propagation efficiency is largely determined by adventitious root (AR) formation at the bases of stem cuttings. However, our understanding of the molecular mechanisms contributing to AR morphogenesis in trees remains limited, despite the importance of vegetative propagation, currently the most common practice for tree breeding and commercialization. Here, we identified Populus-specific miR476a as a regulator of wound-induced adventitious rooting that acts by orchestrating mitochondrial homeostasis. MiR476a exhibited inducible expression during AR formation and directly targeted several Restorer of Fertility like (RFL) genes encoding mitochondrion-localized pentatricopeptide repeat proteins. Genetic modification of miR476a-RFL expression revealed that miR476a/RFL-mediated dynamic regulation of mitochondrial homeostasis influences AR formation in poplar. Mitochondrial perturbation via exogenous application of a chemical inhibitor indicated that miR476a/RFL-directed AR formation depends on mitochondrial regulation that acts via auxin signaling. Our results thus establish a microRNA-directed mitochondrion-auxin signaling cascade required for AR development, providing insights into the role of mitochondrial regulation in the developmental plasticity of plants
Beschreibung:Date Completed 14.05.2021
Date Revised 14.05.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.17252