Genetic Structure of Plasmodiophora brassicae Populations Virulent on Clubroot Resistant Canola (Brassica napus)

Clubroot, caused by Plasmodiophora brassicae Woronin, is a significant threat to the canola (Brassica napus L.) industry in Canada. Clubroot resistance has been overcome in more than 200 fields since 2013, representing one of the biggest challenges to sustainable canola production. The genetic struc...

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Veröffentlicht in:Plant disease. - 1997. - 105(2021), 11 vom: 24. Nov., Seite 3694-3704
1. Verfasser: Askarian, Homa (VerfasserIn)
Weitere Verfasser: Akhavan, Alireza, González, Leonardo Galindo, Hwang, Sheau-Fang, Strelkov, Stephen E
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article Brassica napus Plasmodiophora brassicae SSR markers clubroot genetic diversity
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520 |a Clubroot, caused by Plasmodiophora brassicae Woronin, is a significant threat to the canola (Brassica napus L.) industry in Canada. Clubroot resistance has been overcome in more than 200 fields since 2013, representing one of the biggest challenges to sustainable canola production. The genetic structure of 36 single-spore isolates derived from 12 field isolates of P. brassicae collected before and after the introduction of clubroot resistant (CR) canola cultivars (2005-2014) was evaluated by simple sequence repeat (SSR) marker analysis. Polymorphisms were detected in 32 loci with the identification of 93 distinct alleles. A low level of genetic diversity was found among the single-spore isolates. Haploid linkage disequilibrium and number of migrants suggested that recombination and migration were rare or almost absent in the tested P. brassicae population. A relatively clear relationship was found between the genetic structure and virulence phenotypes of the pathogen as defined on the differential hosts of Somé et al., Williams, and the Canadian Clubroot Differential (CCD) set. Although genetic variability within each pathotype group, as classified on each differential system, was low, significant genetic differentiation was observed among the pathotypes. The highest correlation between genetic structure and virulence was found among matrices produced with genetic data and the hosts of the CCD set, with a threshold index of disease of 50% to distinguish susceptible from resistant reactions. Genetically homogeneous single-spore isolates provided a more complete and clearer picture of the population genetic structure of P. brassicae, and the results suggest some promise for the development of pathotype-specific primers 
650 4 |a Journal Article 
650 4 |a Brassica napus 
650 4 |a Plasmodiophora brassicae 
650 4 |a SSR markers 
650 4 |a clubroot 
650 4 |a genetic diversity 
700 1 |a Akhavan, Alireza  |e verfasserin  |4 aut 
700 1 |a González, Leonardo Galindo  |e verfasserin  |4 aut 
700 1 |a Hwang, Sheau-Fang  |e verfasserin  |4 aut 
700 1 |a Strelkov, Stephen E  |e verfasserin  |4 aut 
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