CHYR1 ubiquitinates the phosphorylated WRKY70 for degradation to balance immunity in Arabidopsis thaliana

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 230(2021), 3 vom: 12. Mai, Seite 1095-1109
1. Verfasser:	Liu, Huanhuan (VerfasserIn)
Weitere Verfasser:	Liu, Bao, Lou, Shangling, Bi, Hao, Tang, Hu, Tong, Shaofei, Song, Yan, Chen, Ningning, Zhang, Han, Jiang, Yuanzhong, Liu, Jianquan
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2021
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't CHYR1 SARD1 WRKY70 immunity phosphorylation Arabidopsis Proteins SARD1 protein, Arabidopsis Transcription Factors WRKY70 protein, Arabidopsis

Beschreibung
Zusammenfassung:	© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation. It is critically important for plants to control the trade-off between normal growth and pathogen immunity. However, the underlying molecular mechanism remains largely unknown. Here we report such a mechanism controlled by WRKY70 and its partner CHYR1 in Arabidopsis. We found that both levels of the WRKY70 target gene SARD1 and the phosphorylated forms of WRKY70 were increased in WRKY70OE plants upon Pst DC3000 infection. Mechanistically, phosphorylation of WRKY70 at Thr22 and Ser34 occurs, which then activates SARD1 expression through binding to a WT box. Phosphorylated WRKY70 is degraded by 26S proteasome via CHYR1 when resuming normal growth after infection. In addition, nonphosphorylated WRKY70 represses SARD1 expression by binding to both W (inhibitory activity site) and WT (active activity site) boxes. The binding of WRKY70 to alternative cis-elements of SARD1 through a phosphorylation-mediated switch controlled by CHYR1 contributes to modulating the balance between immunity and growth
Beschreibung:	Date Completed 14.05.2021 Date Revised 14.05.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.17231