High affinity promoter binding of STOP1 is essential for early expression of novel aluminum-induced resistance genes GDH1 and GDH2 in Arabidopsis

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 72(2021), 7 vom: 29. März, Seite 2769-2789
1. Verfasser:	Tokizawa, Mutsutomo (VerfasserIn)
Weitere Verfasser:	Enomoto, Takuo, Ito, Hiroki, Wu, Liujie, Kobayashi, Yuriko, Mora-Macías, Javier, Armenta-Medina, Dagoberto, Iuchi, Satoshi, Kobayashi, Masatomo, Nomoto, Mika, Tada, Yasuomi, Fujita, Miki, Shinozaki, Kazuo, Yamamoto, Yoshiharu Y, Kochian, Leon V, Koyama, Hiroyuki
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2021
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't ALUMINUM-ACTIVATED MALATE TRANSPORTER 1 (ALMT1) Arabidopsis thaliana GLUTAMATE DEHYDROGENASE (GDH) In silico promoter cis-elements prediction SENSITIVE TO PROTON RHIZOTOXICITY1 (STOP1) Acid soil aluminum tolerance post-translational regulation mehr... promoter analysis transcriptional regulation Arabidopsis Proteins STOP1 protein, Arabidopsis Transcription Factors Aluminum CPD4NFA903 GDH1 protein, Arabidopsis EC 1.4.1.2 GDH2 protein, Arabidopsis Glutamate Dehydrogenase

Beschreibung
Zusammenfassung:	© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Malate efflux from roots, which is regulated by the transcription factor STOP1 (SENSITIVE-TO-PROTON-RHIZOTOXICITY1) and mediates aluminum-induced expression of ALUMINUM-ACTIVATED-MALATE-TRANSPORTER1 (AtALMT1), is critical for aluminum resistance in Arabidopsis thaliana. Several studies showed that AtALMT1 expression in roots is rapidly observed in response to aluminum; this early induction is an important mechanism to immediately protect roots from aluminum toxicity. Identifying the molecular mechanisms that underlie rapid aluminum resistance responses should lead to a better understanding of plant aluminum sensing and signal transduction mechanisms. In this study, we observed that GFP-tagged STOP1 proteins accumulated in the nucleus soon after aluminum treatment. The rapid aluminum-induced STOP1-nuclear localization and AtALMT1 induction were detected in the presence of a protein synthesis inhibitor, suggesting that post-translational regulation is involved in these events. STOP1 also regulated rapid aluminum-induced expression for other genes that carry a functional/high-affinity STOP1-binding site in their promoter, including STOP2, GLUTAMATE-DEHYDROGENASE1 and 2 (GDH1 and 2). However STOP1 did not regulate Al resistance genes which have no functional STOP1-binding site such as ALUMINUM-SENSITIVE3, suggesting that the binding of STOP1 in the promoter is essential for early induction. Finally, we report that GDH1 and 2 which are targets of STOP1, are novel aluminum-resistance genes in Arabidopsis
Beschreibung:	Date Completed 21.05.2021 Date Revised 31.05.2022 published: Print CommentIn: J Exp Bot. 2021 Mar 29;72(7):2269-2272. - PMID 33779752 Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erab031