Urea-based amino sugar agent clears murine liver and preserves protein fluorescence and lipophilic dyes
Five established clearing protocols were compared with a modified and simplified method to determine an optimal clearing reagent for three-dimensionally visualizing fluorophores in the murine liver, a challenging organ to clear. We report successful clearing of whole liver lobes by modification of a...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 70(2021), 2 vom: 25. Feb., Seite 72-80
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| 1. Verfasser: |
Hough, Michelle
(VerfasserIn) |
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| Weitere Verfasser: |
Fenlon, Michael,
Glazier, Alison,
Short, Celia,
Fernandez, Gerardo Esteban,
Xu, Jiabo,
Mahdi, Elaa,
Asahina, Kinji,
Wang, Kasper S |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
3D imaging
bile duct ligation
computational imaging
fluorescence microscopy
lipophilic tracers
liver clearing
optical clearing agents
prominin-1
mehr...
Amino Sugars
Fluorescent Dyes
Urea
8W8T17847W |
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