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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/jpy.13129
|2 doi
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|a pubmed24n1067.xml
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|a (DE-627)NLM320132447
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|a (NLM)33450056
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|a DE-627
|b ger
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Wilson, Samuel T
|e verfasserin
|4 aut
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| 245 |
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|a Evaluation of argon-induced hydrogen production as a method to measure nitrogen fixation by cyanobacteria
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 01.07.2021
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|a Date Revised 01.07.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 Phycological Society of America.
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|a The production of dihydrogen (H2 ) is an enigmatic yet obligate component of biological dinitrogen (N2 ) fixation. This study investigates the effect on H2 production by N2 fixing cyanobacteria when they are exposed to either air or a gas mixture consisting of argon, oxygen, and carbon dioxide (Ar:O2 :CO2 ). In the absence of N2 , nitrogenase diverts the flow of electrons to the production of H2 , which becomes a measure of Total Nitrogenase Activity (TNA). This method of argon-induced hydrogen production (AIHP) is much less commonly used to infer rates of N2 fixation than the acetylene reduction (AR) assay. We provide here a full evaluation of the AIHP method and demonstrate its ability to achieve high-resolution measurements of TNA in a gas exchange flow-through system. Complete diel profiles of H2 production were obtained for N2 fixing cyanobacteria despite the absence of N2 that broadly reproduced the temporal patterns observed by the AR assay. Comparison of H2 production under air versus Ar:O2 :CO2 revealed the efficiency of electron usage during N2 fixation and place these findings in the broader context of cell metabolism. Ultimately, AIHP is demonstrated to be a viable alternative to the AR assay with several additional merits that provide an insight into cell physiology and promise for successful field application
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a acetylene reduction
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| 650 |
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|a cyanobacteria
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|a hydrogen
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|a nitrogen fixation
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|a nitrogenase
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|a nutrients
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|a phytoplankton
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| 650 |
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|a Argon
|2 NLM
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|a 67XQY1V3KH
|2 NLM
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| 650 |
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|a Hydrogen
|2 NLM
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| 650 |
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|a 7YNJ3PO35Z
|2 NLM
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| 650 |
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|a Nitrogenase
|2 NLM
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| 650 |
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|a EC 1.18.6.1
|2 NLM
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| 650 |
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|a Nitrogen
|2 NLM
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| 650 |
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|a N762921K75
|2 NLM
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| 700 |
1 |
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|a Caffin, Mathieu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a White, Angelicque E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Karl, David M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of phycology
|d 1966
|g 57(2021), 3 vom: 01. Juni, Seite 863-873
|w (DE-627)NLM098182994
|x 1529-8817
|7 nnns
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| 773 |
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|g volume:57
|g year:2021
|g number:3
|g day:01
|g month:06
|g pages:863-873
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|u http://dx.doi.org/10.1111/jpy.13129
|3 Volltext
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