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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/nph.17183
|2 doi
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|a pubmed24n1066.xml
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|a (NLM)33438224
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Pařízková, Barbora
|e verfasserin
|4 aut
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| 245 |
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|a New fluorescent auxin probes visualise tissue-specific and subcellular distributions of auxin in Arabidopsis
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 14.05.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors. New Phytologist © 2021 New Phytologist Trust.
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|a In a world that will rely increasingly on efficient plant growth for sufficient food, it is important to learn about natural mechanisms of phytohormone action. In this work, the introduction of a fluorophore to an auxin molecule represents a sensitive and non-invasive method to directly visualise auxin localisation with high spatiotemporal resolution. The state-of-the-art multidisciplinary approaches of genetic and chemical biology analysis together with live cell imaging, liquid chromatography-mass spectrometry (LC-MS) and surface plasmon resonance (SPR) methods were employed for the characterisation of auxin-related biological activity, distribution and stability of the presented compounds in Arabidopsis thaliana. Despite partial metabolisation in vivo, these fluorescent auxins display an uneven and dynamic distribution leading to the formation of fluorescence maxima in tissues known to concentrate natural auxin, such as the concave side of the apical hook. Importantly, their distribution is altered in response to different exogenous stimuli in both roots and shoots. Moreover, we characterised the subcellular localisation of the fluorescent auxin analogues as being present in the endoplasmic reticulum and endosomes. Our work provides powerful tools to visualise auxin distribution within different plant tissues at cellular or subcellular levels and in response to internal and environmental stimuli during plant development
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis
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|a distribution
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|a fluorescent auxin
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|a in vivo visualisation
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|a subcellular localisation
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|a transport
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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|a Indoleacetic Acids
|2 NLM
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| 650 |
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|a Plant Growth Regulators
|2 NLM
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| 700 |
1 |
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|a Žukauskaitė, Asta
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Vain, Thomas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Grones, Peter
|e verfasserin
|4 aut
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| 700 |
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|a Raggi, Sara
|e verfasserin
|4 aut
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| 700 |
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|a Kubeš, Martin F
|e verfasserin
|4 aut
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| 700 |
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|a Kieffer, Martin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Doyle, Siamsa M
|e verfasserin
|4 aut
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| 700 |
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|a Strnad, Miroslav
|e verfasserin
|4 aut
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| 700 |
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|a Kepinski, Stefan
|e verfasserin
|4 aut
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| 700 |
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|a Napier, Richard
|e verfasserin
|4 aut
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|a Doležal, Karel
|e verfasserin
|4 aut
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| 700 |
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|a Robert, Stéphanie
|e verfasserin
|4 aut
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| 700 |
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|a Novák, Ondřej
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 230(2021), 2 vom: 16. Apr., Seite 535-549
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:230
|g year:2021
|g number:2
|g day:16
|g month:04
|g pages:535-549
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|u http://dx.doi.org/10.1111/nph.17183
|3 Volltext
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