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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202004234
|2 doi
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|a pubmed24n1065.xml
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|a eng
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|a Barbaglia, Andrea
|e verfasserin
|4 aut
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|a Mirroring Action Potentials
|b Label-Free, Accurate, and Noninvasive Electrophysiological Recordings of Human-Derived Cardiomyocytes
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|c 2021
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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| 500 |
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|a Date Completed 14.10.2021
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|a Date Revised 14.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a The electrophysiological recording of action potentials in human cells is a long-sought objective due to its pivotal importance in many disciplines. Among the developed techniques, invasiveness remains a common issue, causing cytotoxicity or altering unpredictably cell physiological response. In this work, a new approach for recording intracellular signals of outstanding quality and with noninvasiveness is introduced. By taking profit of the concept of mirror charge in classical electrodynamics, the new proposed device transduces cell ionic currents into mirror charges in a microfluidic chamber, thus realizing a virtual mirror cell. By monitoring mirror charge dynamics, it is possible to effectively record the action potentials fired by the cells. Since there is no need for accessing or interacting with the cells, the method is intrinsically noninvasive. In addition, being based on optical recording, it shows high spatial resolution and high parallelization. As shown through a set of experiments, the presented methodology is an ideal candidate for the next generation devices for the reliable assessment of cardiotoxicity on human-derived cardiomyocytes. More generally, it paves the way toward a new family of in vitro biodevices that will lay a new milestone in the field of electrophysiology
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|a Journal Article
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|a action potential recording
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|a electrophysiology
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|a fluorescence optical recording
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|a in vitro toxicology
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|a Biocompatible Materials
|2 NLM
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|a Silicon Compounds
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|a silicon nitride
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| 700 |
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|a Dipalo, Michele
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1 |
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|a Melle, Giovanni
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| 700 |
1 |
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|a Iachetta, Giuseppina
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| 700 |
1 |
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|a Deleye, Lieselot
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hubarevich, Aliaksandr
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| 700 |
1 |
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|a Toma, Andrea
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| 700 |
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|a Tantussi, Francesco
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|a De Angelis, Francesco
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|t Advanced materials (Deerfield Beach, Fla.)
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|g 33(2021), 7 vom: 30. Feb., Seite e2004234
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|u http://dx.doi.org/10.1002/adma.202004234
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