|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM319675122 |
| 003 |
DE-627 |
| 005 |
20231225171943.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/vcp.12912
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1065.xml
|
| 035 |
|
|
|a (DE-627)NLM319675122
|
| 035 |
|
|
|a (NLM)33403708
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Howell, Margaret
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Multiplex fluorescent immunocytochemistry for the diagnosis of feline infectious peritonitis
|b Determining optimal storage conditions
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 04.03.2021
|
| 500 |
|
|
|a Date Revised 04.03.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2021 American Society for Veterinary Clinical Pathology.
|
| 520 |
|
|
|a BACKGROUND: Feline Infectious Peritonitis (FIP) is a fatal disease of cats that can be very difficult to definitively diagnose antemortem. Multiplex fluorescent immunocytochemical (MF-ICC) assays are emerging as useful diagnostic tests in veterinary medicine, particularly for fluid samples
|
| 520 |
|
|
|a OBJECTIVE: We aimed to develop and optimize an MF-ICC assay to detect feline coronavirus within macrophages, with the primary goal of determining the allowable/recommended sample storage conditions for clinical use of this assay
|
| 520 |
|
|
|a METHODS: A feline macrophage cell line was infected with the FIP virus. Following harvest into EDTA tubes (simulating typical clinical collection of effusion), cells were stored at 4℃, 22℃, and 37℃. For each temperature condition, slides for MF-ICC were made at 0, 1, 2, 3, and 5 days post-collection. To assess the stability of immunoreactivity following fixation, freshly harvested infected cells were fixed onto slides and maintained at 4℃ for 1, 2, 4, and 12 weeks. All slides were analyzed by MF-ICC for the presence of mononuclear cells with co-expression of vimentin and coronaviral antigen
|
| 520 |
|
|
|a RESULTS: MF-ICC confirmed that cells tested positive for coronavirus at 4℃ through 3 days post-harvest, 22℃ through 48 hours post-harvest, and 37℃ through 24 hours post-harvest. The MF-ICC assay was successfully performed on fixed slides through the 12-week time point. This assay also demonstrated positive results on a clinical sample of abdominal fluid from a cat later confirmed to have FIP
|
| 520 |
|
|
|a CONCLUSIONS: The MF-ICC assay described here offers a potentially specific and relatively stable antemortem diagnostic test for feline infectious peritonitis. Evaluation of this assay in clinical samples is ongoing
|
| 650 |
|
4 |
|a Case Reports
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a FIPV
|
| 650 |
|
4 |
|a cat
|
| 650 |
|
4 |
|a coronavirus
|
| 650 |
|
4 |
|a diagnostics
|
| 650 |
|
4 |
|a effusion fluid
|
| 650 |
|
4 |
|a fcwf cells
|
| 700 |
1 |
|
|a Evans, Samantha J M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Cornwall, Michelle
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Santangelo, Kelly S
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 49(2020), 4 vom: 16. Dez., Seite 640-645
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
|
| 773 |
1 |
8 |
|g volume:49
|g year:2020
|g number:4
|g day:16
|g month:12
|g pages:640-645
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/vcp.12912
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 49
|j 2020
|e 4
|b 16
|c 12
|h 640-645
|