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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13324
|2 doi
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|a pubmed25n1063.xml
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|a (NLM)33340371
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|a eng
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| 100 |
1 |
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|a Li, Kexin
|e verfasserin
|4 aut
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| 245 |
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|a Molecular and functional identification of a β-defensin homolog in large yellow croaker (Larimichthys crocea)
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 19.05.2021
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|a Date Revised 19.05.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 John Wiley & Sons Ltd.
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|a β-defensin (BD) is a cysteine-rich cationic antibacterial peptide that is active against a wide range of bacteria. Here, a β-defensin homolog (LcBD2) was identified in large yellow croaker (Larimichthys crocea). The open reading frame of LcBD2 contains 195 nucleotides, encoding a protein of 64 amino acids that possesses a typical arrangement of six conserved cysteine residues (C31 , C37 , C41 , C53 , C59 and C60 ). LcBD2 transcripts were constitutively expressed in all examined tissues and significantly increased in head kidney, spleen and gills by Vibrio alginolyticus. The synthetic LcBD2 peptide imparted antimicrobial effects on both Gram-negative bacteria (V. campbellii, V. parahaemolyticus, V. alginolyticus, V. harveyi and Pseudomonas plecoglossicida) and Gram-positive bacteria (Bacillus subtilis). We also observed that after treatment with synthetic LcBD2 peptide, numerous blisters appeared on the membrane of P. plecoglossicida, which in turn may result in cell membrane breakage and bacterial death. Moreover, the synthetic LcBD2 peptide significantly upregulated the expression levels of TNF-α2, IL-1β and CXCL8_L1 in monocytes/macrophages, while downregulated expression level of IL-10. The LcBD2 peptide also remarkedly enhanced the phagocytosis of monocytes/macrophages. These results indicate that LcBD2 not only protects large yellow croaker against multiple bacterial pathogens but also plays a role in activation of monocytes/macrophages
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|a Journal Article
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|a Pseudomonas plecoglossicida
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|a antibacterial activity
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4 |
|a large yellow croaker
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| 650 |
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4 |
|a monocytes/macrophages
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4 |
|a β-defensin
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| 650 |
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7 |
|a Fish Proteins
|2 NLM
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| 650 |
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7 |
|a beta-Defensins
|2 NLM
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| 700 |
1 |
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|a Li, Wanru
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Xiaojuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luo, Tian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mu, Yinnan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Xinhua
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 44(2021), 4 vom: 19. Apr., Seite 391-400
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnas
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| 773 |
1 |
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|g volume:44
|g year:2021
|g number:4
|g day:19
|g month:04
|g pages:391-400
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|u http://dx.doi.org/10.1111/jfd.13324
|3 Volltext
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|h 391-400
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